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dc.contributor.authorCantanhêde, Lilian Motta
dc.contributor.authorJúnior, Cipriano Ferreira da Silva
dc.contributor.authorIto, Marcos Massayuki
dc.contributor.authorFelipin, Kátia Paula
dc.contributor.authorNicolete, Roberto
dc.contributor.authorSalcedo, Juan Miguel Villalobos
dc.contributor.authorPorrozzi, Renato
dc.contributor.authorCupolillo, Elisa
dc.contributor.authorFerreira, Ricardo de Godoi Mattos
dc.date.accessioned2016-01-21T21:36:45Z
dc.date.available2016-01-21T21:36:45Z
dc.date.issued2015
dc.identifier.citationCANTANHÊDE, Lilian Motta et al. Further Evidence of an Association between the Presence of Leishmania RNAVirus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients. PLoS Negl. Trop. Dis., v. 9, n. 9, p. 4-11, Sept. 15, 2015.
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/12589
dc.language.isoeng
dc.publisherPLOS Neglected Tropical Diseases
dc.rightsopen access
dc.titleFurther Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
dc.typeArticle
dc.identifier.doi10.1371/journal.pntd.0004079
dc.description.abstractenTegumentary Leishmaniasis (TL) is endemic in Latin America, and Brazil contributes approximately 20 thousand cases per year. The pathogenesis of TL, however, is still not fully understood. Clinical manifestations vary from cutaneous leishmaniasis (CL) to more severe outcomes, such as disseminated leishmaniasis (DL), mucosal leishmaniasis (ML) and diffuse cutaneous leishmaniasis (DCL). Many factors have been associated with the severity of the disease and the development of lesions. Recent studies have reported that the presence of Leishmania RNA virus 1 infecting Leishmania (Leishmania RNA virus 1, LRV1) is an important factor associated with the severity of ML in experimental animal models. In the present study, 156 patients who attended Rondonia's Hospital of Tropical Medicine with both leishmaniasis clinical diagnoses (109 CL; 38 ML; 5 CL+ML; 3 DL and 1 DCL) and molecular diagnoses were investigated. The clinical diagnosis were confirmed by PCR by targeting hsp70 and kDNA DNA sequences and the species causing the infection were determined by HSP70 PCR-RFPL. The presence of LVR1 was tested by RT-PCR. Five Leishmania species were detected: 121 (77.6%) samples were positive for Leishmania (Viannia) braziliensis, 18 (11.5%) were positive for Leishmania (V.) guyanensis, 3 (1.8%) for Leishmania (V.) lainsoni, 2 (1.3%) for Leishmania (Leishmania) amazonensis and 2 (1.3%) for Leishmania (V.) shawi. Six (3.9%) samples were positive for Leishmania sp. but the species could not be determined, and 4 (2.6%) samples were suggestive of mixed infection by L. (V.) braziliensis and L. (V.) guyanensis. The virus was detected in L. braziliensis (N = 54), L. guyanensis (N = 5), L. amazonensis (N = 2), L. lainsoni (N = 1) and inconclusive samples (N = 6). Patients presenting with CL+ML, DL and DCL were excluded from further analysis. Association between the presence of the virus and the disease outcome were tested among the remaining 147 patients (CL = 109 and ML = 38). Of them, 71.1% (n = 27) mucosal lesions were positive for LRV1, and 28.9% (n = 11) were negative. In cutaneous lesions, 36.7%(n = 40) were positive and 63.3%(n = 69) were negative for LRV1. The ratio P(ML|LRV1+)/P(ML|LRV1-) was 2.93 (CI95%1.57. . .5.46; p<0.001), thus corroborating the hypothesis of the association between LRV1 and the occurrence of mucosal leishmaniasis, as previously described in animal models; it also indicates that LRV1 is not the only factor contributing to the disease outcome.
dc.creator.affilliationFundação Oswaldo Cruz. Porto Velho, RO, Brasil.
dc.creator.affilliationGoverno do Estado de Rondônia. Secretaria de Saúde. Porto Velho, RO, Brasil.
dc.creator.affilliationGoverno do Estado de Rondônia. Secretaria de Saúde. Porto Velho, RO, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Porto Velho, RO, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Porto Velho, RO, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Porto Velho, RO, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Porto Velho, RO, Brasil.
dc.subject.enLeishmania
dc.subject.enRNA Virus 1
dc.subject.enMucosal Manifestations
dc.subject.enTegumentary
Appears in Collections:IOC - Artigos de Periódicos
RO - Artigos de Periódicos

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