Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12612
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dc.contributor.authorMatos, Najla Benevides
dc.contributor.authorPieri, Fabio A.
dc.contributor.authorPenatti, Marilene
dc.contributor.authorOrlandi, Patrícia P.
dc.date.accessioned2016-01-25T19:36:36Z
dc.date.available2016-01-25T19:36:36Z
dc.date.issued2015
dc.identifier.citationBENEVIDES-MATOS, N. et al. Adherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon. Brazilian Journal of Microbiology (Online), v. 46, p. 131-137, 2015.
dc.identifier.issn1678-4405
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/12612
dc.language.isoeng
dc.publisherBrazilian Journal of Microbiology.
dc.rightsopen access
dc.titleAdherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon.
dc.typeArticle
dc.identifier.doihttp://dx.doi.org/10.1590/S1517-838246120130917
dc.description.abstractenThe bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli. Studies of epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho, Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli. Out of 1,156 isolates obtained, 128 (11.0%) were positive for eae genes corresponding to EPEC, however only 38 (29.6%) of these amplified bfpA gene. EAEC were isolated from 164 (14.1%) samples; of those 41(25%), 32 (19%) and 16 (9.7%) amplified eagg, aggA or aafA genes, respectively and aggA was significantly associated with diarrhoea (P = 0.00006). DAEC identified by their adhesion pattern and there were few isolates. In conclusion, EAEC was the main cause of diarrhoea in children, especially when the aggA gene was present, followed by EPEC and with a negligible presence of DAEC.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais. Porto Velho, RO, Brazil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brazil.
dc.creator.affilliationSecretaria de Estado da Saúde. Hospital Infantil Cosme e Damião. Porto Velho, RO, Brazil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brazil.
dc.subject.enEnteroadherent
dc.subject.enEscherichia coli
dc.subject.endiarrhoea
dc.subject.enchildren
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