Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12708
Title: Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
Authors: Nardy, Ana Flávia F. R.
Silva Filho, João Luiz da
Pérez, Ana Rosa
Meis, Juliana de
Oliveira, Désio Aurélio Farias de
Penha, Luciana
Oliveira, Isadora de Araújo
Dias, Wagner B.
Todeschini, Adriane R.
LIma, Célio Geraldo Freire de
Bellio, Maria
Neves, Celso Caruso
Pinheiro, Ana Acácia
Takiya, Christina Maeda
Bottasso, Oscar
Savino, Wilson
Morrot, Alexandre
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
National University of Rosario. Institute of Immunology. Rosario, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Abstract: In experimental Trypanosoma cruzi infections, severe thymic atrophy leads to release of activated CD4 þ CD8 þ double-positive (DP) T cells to the periphery. In humans, activated DP T cells are found in the blood in association with severe cardiac forms of human chronic Chagas disease. The mechanisms underlying the premature thymocyte release during the chagasic thymic atrophy remain elusive. We tested whether the migratory properties of intrathymic thymocytes are modulated by the parasite trans-sialidase (TS). We found that TS affected the dynamics of thymocytes undergoing intrathymic maturation, and these changes were accompanied by an increase in the number of recent DP thymic emigrants in the peripheral lymphoid organs. We demonstrated that increased percentages of blood DP T cell subsets were associated with augmented antibody titers against TS in chagasic patients with chronic cardiomyopathy. In vitro studies showed that TS was able to activate the MAPK pathway and actin filament mobilization in thymocytes. These effects were correlated with its ability to modulate the adhesion of thymocytes to thymic epithelial cells and their migration toward extracellular matrix. These findings point to effects of TS that could influence the escape of immature thymocytes in Chagas disease.
Keywords: Thymocyte
Chagas Disease
Trypanosoma cruzi
DeCS: Doença de Chagas
Trypanosoma cruzi
Movimento Celular
Issue Date: 2013
Publisher: Elsevier
Citation: NARDY, Ana Flávia F. R. et al. Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes. Microbes and Infection, v. 15, p.365e374, 2013.
DOI: 10.1016/j.micinf.2013.02.003
ISSN: 1286-4579
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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