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CONTRASTING GENETIC STRUCTURE BETWEEN MITOCHONDRIAL AND NUCLEAR MARKERS IN THE DENGUE FEVER MOSQUITO FROM RIO DE JANEIRO: IMPLICATIONS FOR VECTOR CONTROL
Rasic, Gordana et al. | Data do documento: 2015
Autor(es)
Rasic, Gordana
Schama, Renata
Powell, Rossana
Freitas, Rafael Maciel de
Endersby-Harshman, Nancy M.
Filipovic, Igor
Sylvestre, Gabriel
Máspero, Renato C.
Hoffmann, Ary A.
Afiliação
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Secretaria Municipal de Saúde do Rio de Janeiro. Superintendência de Vigilância em Saúde. Coordenação de Vigilância Ambiental em Saúde. Gerência de Risco Biológico. Rio de Janeiro, RJ, Brasil.
The University of Melbourne. Bio21 Institute. School of Biosciences. Pest and Environmental Adaptation Research Group. Parkville, Vic, Australia.
Resumo em Inglês
Dengue is the most prevalent global arboviral disease that affects over 300 million people every year. Brazil has the highest number of dengue cases in the world, with the most severe epidemics in the city of Rio de Janeiro (Rio). The effective control of dengue is critically dependent on the knowledge of population genetic structuring in the primary dengue vector, the mosquito Aedes aegypti. We analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via Restriction-site Associated DNA sequencing, as well as traditional microsatellite markers in Ae. aegypti from Rio. We found four divergent mitochondrial lineages and a strong spatial structuring of mitochondrial variation, in contrast to the overall nuclear homogeneity across Rio. Despite a low overall differentiation in the nuclear genome, we detected strong spatial structure for variation in over 20 genes that have a significantly altered expression in response to insecticides, xenobiotics, and pathogens, including the novel biocontrol agent Wolbachia. Our results indicate that high genetic diversity, spatially unconstrained admixing likely mediated by male dispersal, along with locally heterogeneous genetic variation that could affect insecticide resistance and mosquito vectorial capacity, set limits to the effectiveness of measures to control dengue fever in Rio.
Palavras-chave em inglês
Aedes aegypti
RAD-seq
Rio de Janeiro
Genetic structure
Microsatellites
Mito-nuclear discordance
Vector control
 
DeCS
Aedes
Controle de Vetores
 
Editor
open access
Referência
RASIC, Gordana; et al. Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: implications for vector control. Evolutionary Applications, v.8, n.9, p.901-15, Oct. 2015.
DOI
10.1111/eva.12301
ISSN
1752-4571