Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13223
Title: Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance
Authors: Cantillo-Barraza, Omar
Garcés, Edilson
Gómez-Palacio, Andrés
Cortés, Luis A.
Pereira, André
Marcet, Paula L.
Jansen, Ana M.
Triana-Chávez, Omar
Affilliation: Universidad de Antioquia UdeA. Grupo BCEL. Medellin, Colombia.
Universidad de Antioquia UdeA. Grupo BCEL. Medellin, Colombia.
Universidad de Antioquia UdeA. Grupo BCEL. Medellin, Colombia.
Secretaría de Salud del Departamento de Bolívar. Unidad de Entomología Médica. Cartagena, Colombia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Centers for Disease Control and Prevention (CDC). Division of Parasitic Diseases and Malaria. Entomology Branch. Atlanta, GA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Universidad de Antioquia UdeA. Grupo BCEL. Medellin, Colombia.
Abstract: Background: In Colombia, Rhodnius prolixus and Triatoma dimidiata are the main domestic triatomine species known to transmit T. cruzi. However, there are multiple reports of T. cruzi transmission involving secondary vectors. In this work, we carried out an eco-epidemiological study on Margarita Island, located in the Caribbean region of Colombia, where Chagas disease is associated with non-domiciliated vectors. Methods: To understand the transmission dynamics of Trypanosoma cruzi in this area, we designed a comprehensive, multi-faceted study including the following: (i) entomological evaluation through a community-based insect-surveillance campaign, blood meal source determination and T. cruzi infection rate estimation in triatomine insects; (ii) serological determination of T. cruzi prevalence in children under 15 years old, as well as in domestic dogs and synanthropic mammals; (iii) evaluation of T. cruzi transmission capacity in dogs and Didelphis marsupialis, and (iv) genetic characterization of T. cruzi isolates targeting spliced-leader intergene region (SL-IR) genotypes. Results: Out of the 124 triatomines collected, 94 % were Triatoma maculata, and 71.6 % of them were infected with T. cruzi. Blood-meal source analysis showed that T. maculata feeds on multiple hosts, including humans and domestic dogs. Serological analysis indicated 2 of 803 children were infected, representing a prevalence of 0.25 %. The prevalence in domestic dogs was 71.6 % (171/224). Domestic dogs might not be competent reservoir hosts, as inferred from negative T. cruzi xenodiagnosis and haemoculture tests. However, 61.5 % (8/13) of D. marsupialis, the most abundant synanthropic mammal captured, were T. cruzi-positive on xenodiagnosis and haemocultures. Conclusions: This study reveals the role of peridomestic T. maculata and dogs in T. cruzi persistence in this region and presents evidence that D. marsupialis are a reservoir mediating peridomestic-zoonotic cycles. This picture reflects the complexity of the transmission dynamics of T. cruzi in an endemic area with non-domiciliated vectors where active human infection exists. There is an ongoing need to control peridomestic T. maculata populations and to implement continuous reservoir surveillance strategies with community participation.
Keywords: Colombia
Risk factors for Chagas disease
Trypanosoma cruzi
Seroprevalence
Dogs
Non-domiciliated vectors
DeCS: Doença de Chagas
Fatores de Risco
Trypanosoma cruzi
Soroprevalência
Cães
Issue Date: 2015
Publisher: BioMed Central
Citation: CANTILLO-BARRAZA, Omar; et al. Eco-epidemiological study of an endemic Chagas disease region in northern Colombia reveals the importance of Triatoma maculata (Hemiptera: Reduviidae), dogs and Didelphis marsupialis in Trypanosoma cruzi maintenance. Parasites & Vectors, v.8:482, 10p, 2015.
DOI: 10.1186/s13071-015-1100-2
ISSN: 1756-3305
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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