| Author | Almeida, R. W. de | |
| Author | Espirito Santo, M. P. | |
| Author | Sousa, P. S. F. | |
| Author | Almeida, A. J. de | |
| Author | Lampe, E. | |
| Author | Lewis-Ximenez, L. L. | |
| Access date | 2016-04-05T11:00:44Z | |
| Available date | 2016-04-05T11:00:44Z | |
| Document date | 2015 | |
| Citation | ALMEIDA, R. W. de; et al. Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C. Braz J Med Biol Res, v.48, n.6, p.553-556, June 2015. | pt_BR |
| ISSN | 1414-431X | |
| URI | https://www.arca.fiocruz.br/handle/icict/13523 | |
| Language | eng | pt_BR |
| Publisher | Associação Brasileira de Divulgação Científica | pt_BR |
| Rights | open access | pt_BR |
| Title | Hepatitis B virus DNA stability in plasma samples under short-term storage at 426C | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/1414-431X20144040 | |
| Abstract | We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 426C for external quality assessment
(EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial
dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/
mL. These were incubated at 426C for up to 7 days and then frozen at –706C. Viral load testing for HBV DNA was performed
for all samples using COBAS1 AmpliPrep/COBAS1 TaqMan1 HBV Test (v.2.0, Roche, Switzerland). Results were compared
with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample
collection. Although the results of this study demonstrated a decrease in HBV DNA viral load ranging from 0.005 to 0.30 log(10)
IU/mL after storage at 426C for up to 7 days, these values did not exceed 0.5 log(10), which is the estimated intra-assay
variation for molecular tests. Thus, the insignificant decrease in viral load suggests that shipment of HBV in plasma samples at
temperatures of up to 426C is permissible if they are frozen within 7 days. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | HBV | pt_BR |
| Subject | Stability studies | pt_BR |
| Subject | Real-time PCR | pt_BR |
| DeCS | Vírus da Hepatite B | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
