Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/13968
Title: Differential pulse voltammetric studies on Lamivudine: an antiretroviral drug
Authors: Leandro, Katia Christina
Moreira, Josino Costa
Farias, Pércio Augusto Mardini
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Química. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sérgio Arouca. Rio de Janeiro, RJ, Brasil.
Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil.
Abstract: Lamivudine (also known as 3TC) is a dideoxynucleoside analogue, which undergoes intracellular phosphorylation in the putative active metabolite, lamivudine triphosphate. Lamivudine triphosphate prevents HIV replication by competitively inhibiting viral reverse transcriptase. Lamivudine has been extensively used in the treatment of HIV patients owing to its antiretroviral activity. For the determination of lamivudine in pharmaceuticals, an analytical methodology using voltammetry was developed. Lamivudine was reduced at a hanging mercury drop electrode (HMDE) at –1.16 V vs Ag/AgCl at pH 2.0. The influence of electroanalytical parameters such as scan rate (20 mV·s–1), amplitude (50 mV), nature of the support electrolyte (Clark-Lubs), and pH (2.0) on the voltammetric signal was optimized. Under these op- timized conditions, the method had been validated using pharmaceutical formulations. The lamivudine peak current varied linearly with its concentration from 1.15 to 10.40 mg·L–1, detection and determination limits of 0.46 and 1.0 mg·L–1, respectively, and recovery of 95.15% with a relative standard deviation of 1.10%.
Keywords: Lamivudine Determination
HIV
Antiretroviral Drugs
Voltammetric Analysis
keywords: Lamivudina
HIV
Drogas anti-retrovirais
Voltametria
DeCS: Lamivudina
Fármacos Anti-HIV
Inibidores da Transcriptase Reversa
HIV
Antirretrovirais
Issue Date: 2013
Publisher: Scientific Research Publishing
Citation: LEANDRO, K. C.; MOREIRA, J. C.; FARIAS, P. A. M. Differential pulse voltammetric studies on Lamivudine: an antiretroviral drug. Am. J. Anal. Chem., v. 4, n. 6A, p. 47-51, 2013.
DOI: http://dx.doi.org/10.4236/ajac.2013.46A007
ISSN: 2156-8251
Copyright: open access
Appears in Collections:INCQS - Artigos de Periódicos

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