Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14517
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dc.contributor.authorMartins, Darliane A.
dc.contributor.authorGouvea, Ligiane R.
dc.contributor.authorMuniz, Gabriel S. Vignoli
dc.contributor.authorLouro, Sonia R.W.
dc.contributor.authorBatista, Denise da Gama Jaen
dc.contributor.authorSoeiro, Maria de Nazaré C.
dc.contributor.authorTeixeira, Letícia R.
dc.date.accessioned2016-06-19T21:22:40Z
dc.date.accessioned2016-07-08T12:22:04Z
dc.date.available2016-06-19T21:22:40Z
dc.date.available2016-07-08T12:22:04Z
dc.date.issued2016
dc.identifier.citationMARTINS, Darliane A. et al. Norfloxacin and N-Donor Mixed-Ligand Copper(II) Complexes: Synthesis, Albumin Interaction, and Anti-Trypanosoma cruzi Activity. Bioinorganic Chemistry and Applications, v.2016, Article ID5027404, 11p, 2016.
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/14517
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.rightsopen access
dc.subject.otherNorfloxacino
dc.subject.otherDoença de Chagas
dc.subject.otherTrypanosoma cruzi
dc.subject.otherAlbuminas
dc.titleNorfloxacin and N-Donor Mixed-Ligand Copper(II) Complexes: Synthesis, Albumin Interaction, and Anti-Trypanosoma cruzi Activity
dc.typeArticle
dc.identifier.doi10.1155/2016/5027404
dc.description.abstractenCopper(II) complexes with the first-generation quinolone antibacterial agent norfloxacin containing a nitrogen donor heterocyclic ligand 2,2󸀠-bipyridine (bipy) or 1,10-phenanthroline (phen)were prepared and characterized by IR, EPR spectra,molar conductivity, and elemental analyses.The experimental data suggest that norfloxacin was coordinated to copper(II) through the carboxylato and ketone oxygen atoms. The interaction of the copper(II) complexes with bovine serum albumin (BSA) and human serum albumin (HSA) was investigated using fluorescence quenching of the tryptophan residues and copper(II) EPR spectroscopy. The results of fluorescence titration revealed that copper(II) complexes have a moderate ability to quench the intrinsic fluorescence of the albumins through a static quenching mechanism. EPR experiments showed that BSA and HSA Cu(II) sites compete with NOR for Cu(II)-bipy and Cu(II)-phen to form protein mixed-ligand complexes. Copper(II) complexes, together with the corresponding ligands, were evaluated for their trypanocidal activity in vitro against Trypanosoma cruzi, the causative agent of Chagas disease. The tests performed using bloodstream trypomastigotes showed that the Cu(II)-N-donor precursors and the metal complexes were more active than the free fluoroquinolone.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Departamento de Química. Belo Horizonte, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Departamento de Química. Belo Horizonte, MG, Brasil.
dc.creator.affilliationPontifíıcia Universidade Católica do Rio de Janeiro. Departamento de Física. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationPontifíıcia Universidade Católica do Rio de Janeiro. Departamento de Física. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Departamento de Química. Belo Horizonte, MG, Brasil.
dc.subject.enChagas disease
dc.subject.enTrypanosoma cruzi protozoa
dc.subject.enNorfloxacin
dc.subject.enAnti-Trypanosoma cruzi Activity
dc.identifier.eissn1687-479X
Appears in Collections:IOC - Artigos de Periódicos

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