Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14606
Title: Use of Noninvasive Parameters to Evaluate Swiss Webster Mice During Trypanosoma cruzi Experimental Acute Infection
Authors: Campos, Jerônimo D. S
Hoppe, Luanda Y.
Duque, Thabata L. A.
Castro, Solange Lisboa de
Oliveira, Gabriel M.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Biofilmes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract: Until now, there has been neither an agreed-upon experimental model nor descriptors of the clinical symptoms that occur over the course of acute murine infection. The aim of this work is to use noninvasive methods to evaluate clinical signs in Swiss Webster mice that were experimentally infected with the Y strain of Trypanosoma cruzi during acute phase (Inf group). Infected mice showed evident clinical changes beginning in the second week of infection (wpi) when compared to the noninfected group (NI): (1) animals in hunched postures, closed eyes, lowered ears, peeling skin, increased piloerection, prostration, and social isolation; (2) significant decrease in body weight (Inf: 26.2 6 2.6 g vs. NI: 34.2 6 2.5 g) and in chow (1.5 6 0.3 vs. 6.3 6 0.5 mg) and water (2.4 6 0.5 vs. 5.8 6 0.7 ml) intake; (3) significant decrease of spontaneous activity as locomotor parameters: distance (0.64 6 0.06 vs. 1.8 6 0.13 m), velocity (1.9 6 0.3 vs. 6.7 6 1.5 cm/sec), and exploratory behavior by frequency (1.0 6 0.5 vs. 5.7 6 1.0 events) and duration (1.4 6 0.3 vs. 5.1 6 0.5 sec in central arena region); (4) significant increase in the PR (41.7 6 8.7 vs. 27.6 6 1.9 msec) and QT intervals (39.7 6 2.0 vs. 27.5 6 4.0 msec), and a decreased cardiac frequency (505 6 52.8 vs. 774 6 17.8 msec), showing a marked sinus bradycardia and an atrioventricular block. At 3 and 4 wpi, the surviving animals showed a tendency of recovery in body weight, food intake, locomotor activity, and exploratory interest. Through the use of noninvasive parameters, we were able to monitor the severity of the infection in individuals prior to death. Our perspective is the application of noninvasive methods to describe clinical signs over the course of acute infection complementing the preclinical evaluation of new agents, alone or in combination with benznidazole.
Keywords: Trypanosoma cruzi
Mice
Chagas Disease
Noninvasive Parameters
keywords: Trypanosoma cruzi
Camundongos
Doença de Chagas
Não-invasivos parâmetros
Issue Date: 2016
Publisher: American Society of Parasitologists
DOI: 10.1645/15-884
ISSN: 0022-3395
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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