Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14689
Title: Hairless mice as an experimental model of infection with Leishmania amazonensis
Authors: Araujo, Vanessa Carneiro Pereira
Fukutani, Kiyoshi Ferreira
Oshiro, Elisa Teruya
Rodrigues, Patrik Oening
Rizk, Yasmin Silva
Carollo, Carlos Alexandre
Arruda, Carla Cardozo Pinto
Affilliation: Universidade Federal de Mato Grosso do Sul. Laboratório de Parasitologia Humana. Centro de Ciências Biológicas e da Saúde. Campo Grande, MG, Brasil
Fundação Gonçalo Moniz, Centro de Pesquisas Gonçalo Moniz. Laborat orio de Imunoparasitologia. Salvador, BA, Brasil
Universidade Federal de Mato Grosso do Sul. Laboratório de Parasitologia Humana. Centro de Ciências Biológicas e da Saúde. Campo Grande, MG, Brasil
Universidade Federal de Mato Grosso do Sul. Laborat ório de Tecnologia Farmacêutica. Centro de Ciências Biológicas e da Saúde. Campo Grande, MG, Brasil
Universidade Federal de Mato Grosso do Sul. Laboratório de Parasitologia Humana. Centro de Ciências Biológicas e da Saúde. Campo Grande, MG, Brasil
Universidade Federal de do Sul. Laboratório de Produtos Naturais e Espectrometria de Massas e LaPNEM. Centro de Ciências Biológicas e da Saúde. Campo Grande, MG, Brasil
Universidade Federal de Mato Grosso do Sul. Laboratório de Parasitologia Humana. Centro de Ciências Biológicas e da Saúde. Campo Grande, MG, Brasil
Abstract: HRS/J Hairless mice have been investigated as an experimental model in cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis. The animals were inoculated with 106 promastigotes into the right hind footpad and the course of infection was followed up for 30, 60 and 90 days. BALB/c mice were infected and used as control. Hairless mice were susceptible to L. (L.) amazonensis infection and a progressive increase in number of parasites and footpad thickness was detected over time. Signals of dissemination and visceralization were confirmed by the presence of parasite in the draining lymph node of lesion and spleen, at different times post infection. IL-10 gene expression evaluated by RT-PCR was significantly higher in Hairless mice at 60 days post infection, corroborating the pattern of susceptibility. These results point this inbred strain as a promising susceptible model for the study of experimental infection induced by L. (L.) amazonensis. This model would allow the use of other infection
Keywords: Cutaneous leishmaniasis
Experimental infection
HRS/J mouse
Issue Date: 2015
Publisher: Elsevier
Citation: ARAUJO, V. C. P. et al. Hairless mice as an experimental model of infection with Leishmania amazonensis. Experimental Parasitology, v. 157, p. 138-144, 2015.
ISSN: 0014-4894
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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