Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14695
Full metadata record
DC FieldValue
dc.contributor.authorZingales, Bianca
dc.contributor.authorPereira, Maria Elizabeth S.
dc.contributor.authorOliveira, Riva P.
dc.contributor.authorAlmeida, Katia A.
dc.contributor.authorUmezawa, Eufrosina S.
dc.contributor.authorSouto, Ricardo P.
dc.contributor.authorVargas, Nancy
dc.contributor.authorCano, Maria Isabel
dc.contributor.authorSilveira, José Franco da
dc.contributor.authorNehme, Nédia S.
dc.contributor.authorMorel, Carlos Medicis
dc.contributor.authorBrener, Zigman
dc.contributor.authorMacedo, Andrea
dc.date.accessioned2016-06-30T11:56:13Z
dc.date.accessioned2016-07-07T12:20:50Z
dc.date.available2016-06-30T11:56:13Z
dc.date.available2016-07-07T12:20:50Z
dc.date.issued1997
dc.identifier.citationZINGALES, Bianca et al. Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone Cl Brener. Acta Tropica, v. 68, p. 159-173, 1997.
dc.identifier.issn0001-706X
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/14695
dc.language.isoeng
dc.publisherElsevier
dc.rightsrestricted access
dc.titleTrypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener
dc.typeArticle
dc.identifier.doi10.1016/S0001-706X(97)00088-0
dc.description.abstractenClone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. CL Brener was obtained by cloning procedures from bloodstream trypomastigotes isolated from mice infected with the CL strain. the doubling time of CL Brener epimastigotes cultured at 28 degrees C in liver infusion-tryptose (LIT) medium is 58 +/- 13 h. Differentiation to metacyclic forms is induced by incubation of epimastigotes in LIT-20% Grace's medium. Metacyclics give very low parasitemia in mice, contrary to what is observed for blood forms which promote 100% mortality of the animals with inocula of 5 x 10(3) parasites. CL Brener blood forms are highly susceptible to nifurtimox, benznidazole and ketoconazole. Allopurinol is inefficient in the treatment of mice experimental infection. the clone infects mammalian cultured cells and performs the complete intracellular cycle at 33 and 37 degrees C. the molecular typing of CL Brener has been done by isoenzymatic profiles; sequencing of a 24S alpha ribosomal RNA gene domain and by schizodeme, randomly amplified polymorphic DNA and DNA fingerprinting analyses. for each typing approach the patterns obtained do not change after prolonged parasite subcultivation in LIT medium (up to 100 generations). the stability of the molecular karyotype of the clone was also confirmed.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil.
dc.creator.affilliationUNIFESP. Escola Paulista de Medicina. São Paulo, SP, Brasil.
dc.creator.affilliationUNIFESP. Escola Paulista de Medicina. São Paulo, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
dc.subject.enTrypanosoma cruz
dc.subject.enGenome project
dc.subject.enReference organism
dc.subject.enMolecular typing
dc.subject.enBiological characteristics
dc.subject.decsTrypanosoma cruzi
dc.subject.decsProjeto Genoma
dc.subject.decsTipagem Molecular
dc.subject.decsCaracterísticas biológicas
dc.identifier.eissn0001-706X
Appears in Collections:MG - IRR - Artigos de Periódicos
IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
carlos26_morel_etal_IOC_1997.pdf1.62 MBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.