Author | Deane, Maria P. | |
Author | Sousa, Maria A. | |
Author | Pereira, Neize M. | |
Author | Gonçalves, Antonio M. | |
Author | Momen, Hooman | |
Author | Morel, Carlos Medicis | |
Access date | 2016-07-05T11:26:20Z | pt_BR |
Access date | 2016-07-08T12:21:55Z | |
Available date | 2016-07-05T11:26:20Z | pt_BR |
Available date | 2016-07-08T12:21:55Z | |
Document date | 1984 | pt_BR |
Citation | DEANE, Maria P. et al. Trypanosoma cruzk Inoculation Schedules and Re-isolation Methods Select Individual Strains from Doubly Infected Mice, as Demonstrated by Schizodeme and Zymodeme Analyses. J. Protozool., v.31, n.2, p.276-280, 1984. | pt_BR |
ISSN | 0022-3921 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/14766 | |
Language | eng | pt_BR |
Publisher | Society of Protozoologists | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Ratos duplamente infectados | pt_BR |
Subject in Portuguese | Estirpes individuais | pt_BR |
Subject in Portuguese | Análise zimodema | pt_BR |
Subject in Portuguese | Análise esquizóide | pt_BR |
Title | Trypanosoma cruzk Inoculation Schedules and Re-isolation Methods Select Individual Strains from Doubly Infected Mice, as Demonstrated by Schizodeme and Zymodeme Analyses | pt_BR |
Type | Article | pt_BR |
Abstract | Groups of mice received double infections with the Y and F strains of Trypanosoma cruzi, the first inoculum of either
strain being followed by a second inoculum of the other strain on day 5, 15, 30-40, or 60-65. Parasites were re-isolated from blood
into culture, either directly or with an intermediate passage in gamma-irradiated mice, at intervals between 7 and 35 days after the
second inoculation. Strain identification in the re-isolated material was by electrophoresis of kDNA fragments generated by the EcoRI
restriction endonuclease and by electrophoresis for glucosephosphate isomerase isozymes. Both strains were identified in 22% of reisolates
originating from the experimental mice and only one of them was present in the remaining re-isolates, strain F being the most
frequent. In some instances either Y or F was re-isolated from the same blood source, depending on whether culturing had been preceded
or not by passage through a mouse. These results are certainly related to strain differences in the various aspects of host-parasite
relationship and, possibly, growth rates in culture. The results demonstrate that: (1) more than one strain of T. cruzi can coexist in the
same host; (2) the timing and method of parasite isolation from the vertebrate host act as selective factors, and further passages (in
mice or cultures) may completely eliminate one (or more) strain from originally mixed trypanosome populations, and (3) kDNA
restriction “fingerprints” and isozyme profiles are simple, sensitive, and reliable techniques for strain identification both in single and
mixed preparations. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Schizodeme Analysis | pt_BR |
Subject | Zymodeme Analyses | pt_BR |
Subject | Doubly Infected Mice | pt_BR |
Subject | Individual Strains | pt_BR |