Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14937
Title: Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization
Authors: Vieira, Deusilene
Gauthier, Marie
Nicolete, Larissa Deadame de Figueiredo
Santos, Alcione Oliveira dos
Picelli, Carina
Honda, Eduardo
Paranhos-Baccalà, Glaucia
Vernet, Guy
Salcedo, Juan Miguel Villalobos
Affilliation: Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.
Fondation Mérieux. Emerging Pathogens Laboratory. IFR 128 BioSciences. Gerland, Lyon, France.
Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.
Fondation Mérieux. Emerging Pathogens Laboratory. IFR 128 BioSciences. Gerland, Lyon, France.
Fondation Mérieux. Emerging Pathogens Laboratory. IFR 128 BioSciences. Gerland, Lyon, France.
Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.
Abstract: The Amazon region is considered to be a high endemic area for Hepatitis B Virus (HBV) infections, Rondônia state having the highest prevalence. The aim of this study was to identify molecular genotypes and mutations in the S gene region of HBV viral genomes from 20 patients using a DNA microarray. Results: Serological tests showed that 88% of patients were HBeAg negative, 82% had anti-HBe antibodies and 33% were co-infected with Hepatitis Delta Virus. Sixteen percent of the patients were considered cirrhotic, and 11% have been transfused. The microarray technique identified the genotypes A (4 patients), D (7 patients) and F (7 patients) in 18 samples. Mutations were detected in all 3 genotypes and, overall, A159G, which has been associated with a reduced antigenicity of the virus, was detected most frequently. In genotype A, G119E was the most frequently detected mutation followed by mutations A159G, F134Y, W172C, Y161F and T143S. A159G was detected in all genotype D and F samples followed by mutations T143S, Y161F, N131T, T114S and G119E in genotype D and mutations T143S, Y161F, N131T, T114S and G119E in genotype F. Conclusion: The analysis of mutations repartition among genotypes suggests that some of them are preferentially or exclusively associated with genotype A, D or F. This type of tool is adapted for clinical and therapy monitoring of patient as well as for molecular epidemiology research on HBV.
Keywords: Hepatitis B Virus
Mutant Detection
DNA Microarray
Amazon Region
Issue Date: 2013
Publisher: World Journal of Cardiovascular Diseases
Citation: VIEIRA, Deusilene et al. Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization. World Journal of Cardiovascular Diseases, v. 3, p. 506-511, 2013.
DOI: http://dx.doi.org/10.4236/wjcd.2013.38080
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos

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