| Author | Guimarães, Monick L. | |
| Author | Velarde-Dunois, Ketty G. | |
| Author | Segurondo, David | |
| Author | Morgado, Mariza G. | |
| Access date | 2016-09-15T15:36:31Z | |
| Available date | 2016-09-15T15:36:31Z | |
| Document date | 2012 | |
| Citation | GUIMARÃES, Monick L. et al. The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains. Virology Journal, v.9:19, 7p, 2012. | pt_BR |
| ISSN | 1743-422X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15815 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | HIV-1 | pt_BR |
| Subject in Portuguese | Bolívia | pt_BR |
| Subject in Portuguese | Subtipos | pt_BR |
| Title | The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains | pt_BR |
| Type | Article | pt_BR |
| Abstract | Background: Molecular epidemiological studies of HIV-1 in South America have revealed the occurrence of
subtypes B, F1 and BF1 recombinants. Even so, little information concerning the HIV-1 molecular epidemiology in
Bolivia is available. In this study we performed phylogenetic analyses from samples collected in Bolivia at two
different points in time over a 10 year span. We analyzed these samples to estimate the trends in the HIV subtype
and recombinant forms over time.
Materials and methods: Fifty one HIV-1 positive samples were collected in Bolivia over two distinct periods (1996
and 2005). These samples were genetically characterized based on partial pol protease/reverse transcriptase (pr/rt)
and env regions. Alignment and neighbor-joining (NJ) phylogenetic analyses were established from partial env (n =
37) and all pol sequences using Mega 4. The remaining 14 env sequences from 1996 were previously characterized
based on HMA-env (Heteroduplex mobility assay). The Simplot v.3.5.1 program was used to verify intragenic
recombination, and SplitsTree 4.0 was employed to confirm the phylogenetic relationship of the BF1 recombinant
samples.
Results: Phylogenetic analysis of both env and pol regions confirmed the predominance of “pure” subtype B
(72.5%) samples circulating in Bolivia and revealed a high prevalence of BF1 genotypes (27.5%). Eleven out of 14
BF1 recombinants displayed a mosaic structure identical or similar to that described for the CRF12_BF variant, one
sample was classified as CRF17_BF, and two others were F1pol/Benv. No “pure” HIV-1 subtype F1 or B” variant of
subtype B was detected in the present study. Of note, samples characterized as CRF12_BF-related were depicted
only in 2005.
Conclusion: HIV-1 genetic diversity in Bolivia is mostly driven by subtype B followed by BF1 recombinant strains
from the CRF12_BF “family”. No significant temporal changes were detected between the mid-1990s and the mid-
2000s for subtype B (76.2% vs 70.0%) or BF1 recombinant (23.8% vs 30.0%) samples from Bolivia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Policía Nacional. La Paz, Bolivia. | pt_BR |
| Affilliation | Ministerio de la Salud. Programa Departamental de ITS, VIH/SIDA. La Paz, Bolivia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | HIV-1 | pt_BR |
| Subject | Subtypes | pt_BR |
| Subject | Bolivia | pt_BR |
| Subject | CRF | pt_BR |
| e-ISSN | 1743-422X | |
