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2030-01-01
Sustainable Development Goals
11 Cidades e comunidades sustentáveisCollections
- IOC - Artigos de Periódicos [12776]
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SYNERGIC EFFECT OF METRONIDAZOLE AND PYRANTEL PAMOATE ON GIARDIA LAMBLIA
Author
Affilliation
Universidade do Estado do Rio de Janeiro. Depto. de Histologia e Embriologia. Laboratório de Microscopia e Processamento de Imagens. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Depto. de Histologia e Embriologia. Laboratório de Microscopia e Processamento de Imagens. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Depto. de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Depto. de Histologia e Embriologia. Laboratório de Microscopia e Processamento de Imagens. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Depto. de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Abstract
Giardia lamblia is a pathogenic protozoan presenting as the main characteristic, the trophozoite capacity to
adhere in host intestinal epithelium, infecting mammals, including humans. The clinical treatment of this
disease is based on metronidazole (Mz) that acts as an alternative electron acceptor, and its reduction
promotes DNA impairment. In veterinary treatment, one of the best options is pyrantel pamoate (Pm), which
the mode of action has not elucidated yet. Different strategies for Giardia treatment have been explored to
avoid side effects to the host. In this context, the efficiency of treatment combining drugs raise as an
interesting alternative for protozoan diseases. Here, we evaluated in vitro synergic effect of Mz and Pm on
trophozoites and on its adherence to IEC-6 cells. The treatment with Mz or Pm was effective on trophozoites,
with IC50/24 h values of 5.3± 0.9 μM and 13.8± 1.4 μM, respectively. The treatment of trophozoites with
different combinations of Mz and Pm were also evaluated, as showed by fractional inhibitory concentration
index (FICI) under 0.5 in all conditions tested, corresponding to the synergic effect. This synergic activity was
also observed when the combinations of 5.3 μM Mz+ 0.4 μM Pm and 13.8 μM Pm+ 0.1 μM Mz induced a
remarkable reduction in % adhesion (85–90% and 52–59%, respectively) and in number of adhered parasites
per 100 cells. The low cytotoxicity to the host cells of the combinations, associated to the strong synergic
potential of the combination, encourage us to further investigate its effect in in vivo models.
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