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A NEXT-GENERATION PROTEOME ARRAY FOR SCHISTOSOMA MANSONI
Assis, Rafael Ramiro de et al. | Fecha del documento: 2016
Autor
Assis, Rafael Ramiro de
Ludolf, Fernanda
Nakajima, Rie
Jasinskas, Al
Oliveira, Guilherme Correa de
Felgner, Philip L.
Jangola, Soraya Torres Gaze
Loukas, Alex
LoVerde, Philip T.
Bethony, Jeffrey Michael
Oliveira, Rodrigo Correa de
Silva, Carlos Eduardo Calzavara
Afiliación
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/ University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA
Universidade Federal de Minas Gerais. Faculdade de Medicina. Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil
University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA
University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA
Vale Technology Institute of Sustainable Development. Belém, PA, Brazil
University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil
James Cook University. Australian Institute of Tropical Health & Medicine. Centre for Biodiscovery and Molecular Development of Therapeutics. Cairns, QLD, Australia
University of Texas Health Science Center. School of Medicine. Departments of Biochemistry and Pathology. San Antonio, TX , USA
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/The George Washington University. School of Medicine and Health Science. Department of Microbiology, Immunology, and Tropical Medicine. Washington, DC, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/ University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA
Resumen en ingles
A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.
Palabras clave en portugues
anticorpos
proteomes
 
Palabras clave en ingles
Antibody signature
Protein microarray
Proteome
Schistosoma mansoni
 
Editor
Elsevier Ltd.
Referencia
ASSIS, Rafael Ramiro de Assis, et al. A next-generation proteome array for Schistosoma mansoni. Int J Parasitol., v. 46, n. 7, p. 411-5, 2016.
DOI
10.1016/j.ijpara.2016.04.001
ISSN
0020-7519