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RUSSIAN "SUCCESSFUL" CLONE B0/W148 OF MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE: A MULTIPLEX PCR ASSAY FOR RAPID DETECTION AND GLOBAL SCREENING
Mokrousov, Igor et al. | Date Issued: 2012
Author
Mokrousov, Igor
Narvskaya, Olga
Vyazovaya, Anna
Otten, Tatiana
Jiao, Wei-Wei
Gomes, Lia Lima
Suffys, Philip N.
Shen, A-Dong
Vishnevsky, Boris
Affilliation
St. Petersburg Pasteur Institute. Laboratory of Molecular Microbiology. St. Petersburg, Russia.
St. Petersburg Pasteur Institute. Laboratory of Molecular Microbiology. St. Petersburg, Russia.
St. Petersburg Pasteur Institute. Laboratory of Molecular Microbiology. St. Petersburg, Russia.
The Research Institute of Phthisiopulmonology. Laboratory of Microbiology. St. Petersburg, Russia.
National Key Discipline of Paediatrics. Key Laboratory of Major Diseases in Children (Capital Medical University). Ministry of Education, Beijing Pediatric Research Institute. Beijing Children’s Hospital affiliated to Capital Medical University, Beijing, China.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.
National Key Discipline of Paediatrics. Key Laboratory of Major Diseases in Children (Capital Medical University). Ministry of Education, Beijing Pediatric Research Institute. Beijing Children’s Hospital affiliated to Capital Medical University, Beijing, China.
The Research Institute of Phthisiopulmonology. Laboratory of Microbiology. St. Petersburg, Russia.
Abstract
We describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a "successful" clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.
Keywords in Portuguese
Reação em Cadeia da Polimerase
Mycobacterium tuberculosis
Beijing genótipo
Russia
 
Keywords
Mycobacterium tuberculosis
Beijing Genotype
Russia
multiplex PCR assay
 
Publisher
American Society for Microbiology
Citation
MOKROUSOV, Igor; et al. Russian “Successful” Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening. Journal of Clinical Microbiology, v.50, n.11, p.3757-3759, Nov. 2012.
DOI
10.1128/JCM.02001-12
ISSN
0095-1137