Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16237
Title: Expression of non-acetylatable lysines 10 and 14 of histone H4 impairs transcription and replication in Trypanosoma cruzi
Authors: Ramos, Thiago Cesar Prata
Nunes, Vinicius Santana
Nardelli, Sheila Cristina
Pascoalino, Bruno dos Santos
Moretti, Nilmar Silvio
Rocha, Antonio Augusto
Augusto, Leonardo da Silva
Schenkman, Sergio
Affilliation: Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Abstract: The histone H4 from Trypanosomatids diverged from other eukaryotes in the N-terminus, a region that undergoes post-translation modifications involved in the control of gene expression, DNA replication, and chromatin assembly. Nonetheless, the N-terminus of Trypanosoma cruzi histone H4 is mainly acetylated at lysine 4. The lysines 10 and 14 are also acetylated, although at less extent, increasing during the S-phase or after DNA damage, which suggests a regulatory function. Here, we investigated the roles of these acetylations by expressing non-acetylated forms of histone H4 in T. cruzi. We found that histone H4 containing arginines at positions 10 or 14, to prevent acetylation were transported to the nucleus and inserted into the chromatin. However, their presence, even at low levels, interfered with DNA replication and transcription, causing a significant growth arrest of the cells. The absence of acetylation also increased the amount of soluble endogenous histones H3 and H4 and affected the interaction with Asf1, a histone chaperone. Therefore, acetylation of lysines 10 and 14 of the histone H4 in trypanosomes could be required for chromatin assembly and/or remodeling required for transcription and replication.
Keywords: Trypanosoma cruzi
Histone
Acetylation
Transcription
Replication
Chromatin
Issue Date: 2015
Publisher: P.T. LoVerde, K.R. Matthews, M. Parsons, A.P. Waters
Citation: RAMOS, Thiago Cesar Prata et al. Expression of non-acetylatable lysines 10 and 14 of histone H4 impairs transcription and replication in Trypanosoma cruzi Molecular & Biochemical Parasitology, v.204, n.1, p. 1-10, nov. 2015.
DOI: 10.1016/j.molbiopara.2015.11.001
ISSN: 0166-6851
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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