Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/16427
Title: Association between the thrombophilic polymorphisms MTHFR C677T, Factor V Leiden, and prothrombin G20210A and recurrent miscarriage in Brazilian women
Authors: Gonçalves, Rozana Oliveira
Fraga, Lucas Rosa
Santos, Wendell Vilas Boas
Carvalho, Acácia Fernandes Lacerda de
Cerqueira, Bruno Antonio Veloso
Sarno, Manoel Alfredo Curvelo
Toralles, Maria Betania P
Vieira, M J
Dutra, C G
Schüler-Faccini, Lavínia
Sanseverino, Maria Teresa Vieira
Gonçalves, Marilda de Souza
Vianna, Fernanda Sales Luiz
Costa, Olivia Lucia Nunes
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil
Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brasil
Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brasil / Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, Brasil
Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil
Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Abstract: Some cases of recurrent first trimester miscarriage have a thrombotic etiology. The aim of this study was to investigate the prevalence of the most common thrombophilic mutations - factor V (FV) Leiden G1691A (FVL), prothrombin (FII) G20210A, and methylenetetrahydrofolate reductase (MTHFR) C677T - in women with recurrent miscarriages. In this case-control study, we included 137 women with two or more consecutive first-trimester miscarriages (£12 weeks of gestation) and 100 healthy women with no history of pregnancy loss, and with at least one living child. DNA was extracted from the patient samples, and the relevant genes (FVL, FII, and MTHFR) were amplified by PCR, followed by restriction fragment length polymorphism, to assess the polymorphisms in these genes. The allelic frequencies of polymorphisms were not significantly different between the case and control groups. Polymorphisms in the MTHFR, FVL, and FII genes were not associated with recurrent miscarriage during the first trimester of pregnancy in Brazilian women (P = 0.479; P = 0.491 and P = 0.107, respectively). However, the etiologic identification of genetic factors is important for genetic counseling.
Keywords: Recurrent miscarriage
First trimester miscarriage
Factor V Leiden
Methylenetetrahydrofolate reductase C677T
FII G20210A
keywords: Aborto
Protrombina
Polimorfismo
Trombose
Humanos
Feminino
Aconselhamento genético
Issue Date: 2016
Publisher: Fundação Norte-Rio-Grandense de Pesquisa e Cultura
Citation: GONÇALVES, R. O. et. al. Association between the thrombophilic polymorphisms MTHFR C677T, Factor V Leiden, and prothrombin G20210A and recurrent miscarriage in Brazilian women. Genetics and Molecular Research, v. 15, n. 3, p. gmr.15038156, 2016.
DOI: 10.4238/gmr.15038156
ISSN: 1676-5680
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Gonçalves RO Association between....pdf336.58 kBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.