| Author | Oliveira, Edward José de | |
| Author | Saliba, Soraya Wilke | |
| Author | Andrade, Camila Filizzola de | |
| Author | Rabello, Ana Lucia Teles | |
| Access date | 2017-01-19T15:27:54Z | |
| Available date | 2017-01-19T15:27:54Z | |
| Document date | 2011 | |
| Citation | OLIVEIRA, Edward José de et al. Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg., v. 105, n. 7, p. 414-416, 2011. | pt_BR |
| ISSN | 0035-9203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16898 | |
| Language | eng | pt_BR |
| Publisher | Royal Society of Tropical Medicine and Hygiene | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | leishmaniose visceral | pt_BR |
| Title | Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.trstmh.2011.04.010 | |
| Abstract | In this study, the direct agglutination test (DAT), using 2-mercaptoethanol (2-ME), kaolin or N-acetylcysteine (NAC) as sample diluents, was used to assay 89 samples from visceral leishmaniasis (VL) patients and 130 samples from patients with other diseases and healthy individuals. Maintaining a cut-off of 1:100, the DAT assays with 2-ME, kaolin or NAC presented sensitivities of 94.4%, 95.5% and 100% (P = 0.09) and specificities of 99.2%, 100% and 97.7% (P = 0.17), respectively. Based on these results, we suggest that NAC can be used as a replacement for 2-ME in the DAT, increasing biosafety in the diagnosis of VL. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Visceral leishmaniasis | pt_BR |
| Subject | Serological diagnosis | pt_BR |
| Subject | DAT | pt_BR |
| Subject | Kaolin suspension | pt_BR |
| Subject | NAC | pt_BR |
| Embargo date | 2030-01-01 | |
