Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17716
Title: Immunoglobulin G response to mammalian cell entry 1A (Mce1A) protein as biomarker of active tuberculosis
Authors: Takenami, Iukary Oliveira
Oliveira, Carolina Cavalcante de
Lima, Filipe R
Soares, Jéssica
Machado, Almério
Riley, Lee W
Arruda, Sérgio Marcos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Secretaria da Saúde do Estado da Bahia. Hospital Especializado Oct avio Mangabeira. Salvador, BA, Brasil
University of California. School of Public Health. Division of Infectious Diseases and Vaccinology. Li Ka Shing Bldg, Berkeley, CA, USA
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Abstract: Cell wall components are major determinants of virulence of Mycobacterium tuberculosis and they contribute to the induction of both humoral and cell-mediated immune response. The mammalian cell entry protein 1A (Mce1A), in the cell wall of M. tuberculosis, mediates entry of the pathogen into mammalian cells. Here, we examined serum immunoglobulin levels (IgA, IgM and total IgG) against Mce1A as a potential biomarker for diagnosis and monitoring tuberculosis (TB) treatment response. Serum samples of 39 pulmonary TB patients and 65 controls (15 healthy household contacts, 19 latently infected household contacts, 13 non-TB and 18 leprosy patients) were screened by ELISA. The median levels of all immunoglobulin classes were significantly higher in TB patients when compared with control groups. The positive test results for IgA, IgM and total IgG were 62, 54 and 82%, respectively. For comparison, routine sputum smear examination diagnosed only 26 (67%) of 39 TB cases. Sensitivities of IgA, IgM and IgG test were 59, 51.3 and 79.5%, respectively, while the specificities observed were 77.3, 83.3 and 84.4%, respectively. A significant decrease compared with baseline was also shown after TB treatment. These results suggest that circulating total IgG antibody to Mce1A could be a complementary tool to diagnosis pulmonary TB.
Keywords: Pulmonary tuberculosis
Mce1A protein
Serodiagnosis
Antibodies
Monitoring
Treatment
keywords: Tuberculose pulmonar
Proteína Mce1A
Serodiagnóstico
Anticorpos
Monitorização
Tratamento
Issue Date: 2016
Publisher: Elsevier
Citation: TAKENAMI, I. O. et al. Immunoglobulin G response to mammalian cell entry 1A (Mce1A) protein as biomarker of active tuberculosis. Tuberculosis, v. 100, p. 82e88, 2016.
DOI: 10.1016/j.tube.2016.07.012
ISSN: 1472-9792
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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