Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/17832
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dc.contributor.authorOliveira, Edson R. A.-
dc.contributor.authorGonçalves, Antônio J. S.-
dc.contributor.authorCosta, Simone M.-
dc.contributor.authorAzevedo, Adriana S.-
dc.contributor.authorMantuano-Barradas, Marcio-
dc.contributor.authorNogueira, Ana Cristina M. A.-
dc.contributor.authorAlves, Ada M. B.-
dc.date.accessioned2017-02-16T11:51:04Z-
dc.date.available2017-02-16T11:51:04Z-
dc.date.issued2016-
dc.identifier.citationOLIVEIRA, Edson R. A. et al. Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route. . PLoS ONE, v.11, n.9, e0163240, 19p, Sept. 2016.pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://www.arca.fiocruz.br/handle/icict/17832-
dc.language.isoengpt_BR
dc.publisherPublic Library of Sciencept_BR
dc.rightsopen accesspt_BR
dc.subject.otherDenguept_BR
dc.subject.otherSaúde Públicapt_BR
dc.subject.otherVacina de DNApt_BR
dc.subject.otherImunidade mediada por células Tpt_BR
dc.titleAspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Routept_BR
dc.typeArticlept_BR
dc.identifier.doi10.1371/journal.pone.0163240-
dc.description.abstractenDengue disease has emerged as a major public health issue across tropical and subtropical countries. Infections caused by dengue virus (DENV) can evolve to life-threatening forms, resulting in about 20,000 deaths every year worldwide. Several animal models have been described concerning pre-clinical stages in vaccine development against dengue, each of them presenting limitations and advantages. Among these models, a traditional approach is the inoculation of a mouse-brain adapted DENV variant in immunocompetent animals by the intracerebral (i.c.) route. Despite the historical usage and relevance of this model for vaccine testing, little is known about the mechanisms by which the protection is developed upon vaccination. To cover this topic, a DNA vaccine based on the DENV non-structural protein 1 (pcTPANS1) was considered and investigations were focused on the induced T cell-mediated immunity against i.c.-DENV infection. Immunophenotyping assays by flow cytometry revealed that immunization with pcTPANS1 promotes a sustained T cell activation in spleen of i.c.-infected mice. Moreover, we found that the downregulation of CD45RB on T cells, as an indicator of cell activation, correlated with absence of morbidity upon virus challenge. Adoptive transfer procedures supported by CFSE-labeled cell tracking showed that NS1-specific T cells induced by vaccination, proliferate and migrate to peripheral organs of infected mice, such as the liver. Additionally, in late stages of infection (from the 7th day onwards), vaccinated mice also presented reduced levels of circulating IFN-γ and IL-12p70 in comparison to non-vaccinated animals. In conclusion, this work presented new aspects about the T cell-mediated immunity concerning DNA vaccination with pcTPANS1 and the i.c. infection model. These insights can be explored in further studies of anti-dengue vaccine efficacy.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clìnica. Rio de Janeiro, RJ, Brasil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.pt_BR
dc.subject.enDenguept_BR
dc.subject.enPublic healthpt_BR
dc.subject.enDNA vaccinept_BR
dc.subject.enT Cell-Mediated Immunitypt_BR
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