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A TRYPANOSOMATID IRON TRANSPORTER THAT REGULATES MITOCHONDRIAL FUNCTION IS REQUIRED FOR LEISHMANIA AMAZONENSIS VIRULENCE
Leishmaniose
Seqüência de aminoácidos
Modelos de doenças
Microscopia eletrônica
Mitocôndria
Virulência
Leishmaniasis
Amino acid sequence
Disease models, animal
Microscopy, electron
Mitochondria
Virulence
Author
Affilliation
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA / Universidade Federal do Ceará. Faculdade de Medicina. Setor Parasitologia. Fortaleza, CE, Brasil
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA / Universidade Federal do Ceará. Faculdade de Medicina. Setor Parasitologia. Fortaleza, CE, Brasil
University of Maryland, College Park. Department of Cell Biology and Molecular Genetics. Maryland, USA
Abstract
Iron, an essential co-factor of respiratory chain proteins, is critical for mitochondrial function and maintenance of its redox balance. We previously reported a role for iron uptake in differentiation of Leishmania amazonensis into virulent amastigotes, by a mechanism that involves reactive oxygen species (ROS) production and is independent of the classical pH and temperature cues. Iron import into mitochondria was proposed to be essential for this process, but evidence supporting this hypothesis was lacking because the Leishmania mitochondrial iron transporter was unknown. Here we describe MIT1, a homolog of the mitochondrial iron importer genes mrs3 (yeast) and mitoferrin-1 (human) that is highly conserved among trypanosomatids. MIT1 expression was essential for the survival of Trypanosoma brucei procyclic but not bloodstream forms, which lack functional respiratory complexes. L. amazonensis LMIT1 null mutants could not be generated, suggesting that this mitochondrial iron importer is essential for promastigote viability. Promastigotes lacking one LMIT1 allele (LMIT1/Δlmit1) showed growth defects and were more susceptible to ROS toxicity, consistent with the role of iron as the essential co-factor of trypanosomatid mitochondrial superoxide dismutases. LMIT1/Δlmit1 metacyclic promastigotes were unable to replicate as intracellular amastigotes after infecting macrophages or cause cutaneous lesions in mice. When induced to differentiate axenically into amastigotes, LMIT1/Δlmit1 showed strong defects in iron content and function of mitochondria, were unable to upregulate the ROS-regulatory enzyme FeSOD, and showed mitochondrial changes suggestive of redox imbalance. Our results demonstrate the importance of mitochondrial iron uptake in trypanosomatid parasites, and highlight the role of LMIT1 in the iron-regulated process that orchestrates differentiation of L. amazonensis into infective amastigotes.
Keywords in Portuguese
LeishmaniaLeishmaniose
Seqüência de aminoácidos
Modelos de doenças
Microscopia eletrônica
Mitocôndria
Virulência
Keywords
LeishmaniaLeishmaniasis
Amino acid sequence
Disease models, animal
Microscopy, electron
Mitochondria
Virulence
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