| Author | Bobosha, Kidist | |
| Author | van der Ploeg-van Schip, Jolien J. | |
| Author | Esquenazi, Danuza A. | |
| Author | Guimarães, Marjorie M. | |
| Author | Martins, Marcia V. | |
| Author | Bekele, Yonas | |
| Author | Fantahun, Yonas | |
| Author | Aseffa, Abraham | |
| Author | Franken, Kees L. M. C. | |
| Author | Gismondi, Ronaldo C. | |
| Author | Pessolani, Maria C. V. | |
| Author | Ottenhoff, Tom H. M. | |
| Author | Pereira, Geraldo M. B. | |
| Author | Geluk, Annemieke | |
| Access date | 2017-04-11T17:08:07Z | |
| Available date | 2017-04-11T17:08:07Z | |
| Document date | 2012 | |
| Citation | BOBOSHA, Kidist; et al. Peptides Derived fromMycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls. Journal of Tropical Medicine, Volume 2012, Article ID132049, 11p, 2012. | pt_BR |
| ISSN | 1687-9686 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18383 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Peptídeos | pt_BR |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Controles endêmicos | pt_BR |
| Title | Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1155/2012/132049 | |
| Abstract | The stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT. Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission. M. leprae antigens that induce T-cell responses in M. leprae exposed and/or infected individuals thus are major targets for new diagnostic tools. Previously, we showed that ML1601c was immunogenic in patients and healthy household contacts (HHC). However, some endemic controls (EC) also recognized this protein. To improve the diagnostic potential, IFN-γ responses to ML1601c peptides were assessed using PBMC from Brazilian leprosy patients and EC. Five ML1601c peptides only induced IFN-γ in patients and HHC. Moreover, 24-hour whole-blood assay (WBA), two ML1601c peptides could assess the level of M. leprae exposure in Ethiopian EC. Beside IFN-γ, also IP-10, IL-6, IL-1β, TNF-α, and MCP-1 were increased in EC from areas with high leprosy prevalence in response to these ML1601c peptides. Thus, ML1601c peptides may be useful for differentiating M. leprae exposed or infected individuals and can also be used to indicate the magnitude of M. leprae transmission even in the context of various HLA alleles as present in these different genetic backgrounds. | pt_BR |
| Affilliation | LUMC. Department of Infectious Diseases. Leiden, The Netherlands / Armauer Hansen Research Institute. Leprosy Section. Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | LUMC. Department of Infectious Diseases. Leiden, The Netherlands | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil / Universidade do Estado do rio de Janeiro. Escola de Ciências Médicas. Rio de Janeiro, RJ, Brasil.. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil | pt_BR |
| Affilliation | Armauer Hansen Research Institute. Leprosy Section. Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | Armauer Hansen Research Institute. Leprosy Section. Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | Armauer Hansen Research Institute. Leprosy Section. Addis Ababa, Ethiopia. | pt_BR |
| Affilliation | LUMC. Department of Infectious Diseases. Leiden, The Netherlands | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Escola de Ciências Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | LUMC. Department of Infectious Diseases. Leiden, The Netherlands. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil / Universidade do Estado do rio de Janeiro. Escola de Ciências Médicas. Rio de Janeiro, RJ, Brasil.. | pt_BR |
| Affilliation | LUMC. Department of Infectious Diseases. Leiden, The Netherlands. | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Peptides | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | Endemic Controls | pt_BR |
| e-ISSN | 1687-9694 | |
