Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18482
Title: Comparative zymographic analysis of metallopeptidase of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis isolates from Peru
Authors: Reyes-Uribe, Patrícia
Santos, Taissa Pereira dos
Jesus, Jose B. de
Rodrigues, Camila Mesquita
Arevalo, Jorge
Cupolillo, Elisa
Cuervo, Patricia
Affilliation: Universidad Peruana Cayetano Heredia. Laboratório de Biologia Molecular de Tripanosomatídeos. Lima, Peru.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.
Universidade Federal de São João del Rei. Departamento de Engenharia de Biossistemas. São João del Rei, MG, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Universidad Peruana Cayetano Heredia. Laboratório de Biologia Molecular de Tripanosomatídeos. Lima, Peru / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
Universidad Peruana Cayetano Heredia. Laboratório de Biologia Molecular de Tripanosomatídeos. Lima, Peru.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.
Abstract: American tegumentary leishmaniasis (ATL) in Peru is mainly associated with Leishmania (Viannia) peruviana and L. (V.) braziliensis. These parasites are genetically related, and their characterization as distinct species is controversial. Despite their genetic similarity, each species is associated with different clinical manifestations of ATL; L. (V.) peruviana causes only cutaneous leishmaniasis, whereas L. (V.) braziliensis can cause both cutaneous and mucocutaneous leishmaniasis. Because the primary cutaneous lesions caused by infection with these species are indistinguishable, it is necessary to develop a suitable method to differentiate them in order to prevent possible metastasis to oropharyngeal mucosa. In the present study, we investigated the proteolytic profile of L. (V.) peruviana and L. (V.) braziliensis isolates from Peru by zymographic analysis in SDS-PAGE copolymerized with gelatin. Enzymes were characterized according to their pH range of activity and sensitivity to distinct peptidase inhibitors. We observed that L. (V.) peruviana isolates displayed three proteolytic bands with molecular masses ranging from 55 to 80 kDa, whereas L. (V.) braziliensis isolates showed six proteolytic activities between 55 and 130 kDa. Using specific inhibitors, we determined that these proteolytic activities are due to metallopeptidases and present optimal activity between the pH range 5.5 and 10.0. Our results suggest that the expression of metallopeptidases in L. (V.) peruviana and L. (V.) braziliensis isolates from Peru is species-specific.
Keywords: Leishmania (Viannia) braziliensis
Leishmania (Viannia) peruviana
Peptidases
Metallopeptidases
keywords: Leishmania (Viannia) braziliensis
Leishmania (Viannia) peruviana
Peptidases
Metalopeptidases
DeCS: Peptídeo Hidrolases
Issue Date: 2012
Publisher: Elsevier
Citation: REYES-URIBE, Patrícia; et al. Comparative zymographic analysis of metallopeptidase of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis isolates from Peru. Parasitology International, v.61, n.4, p.513-519, Dec. 2012.
DOI: 10.1016/j.parint.2012.03.007
ISSN: 1383-5769
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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