Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18828
Title: Leptospirosis diagnosis by immunocapture polymerase chain reaction: a new tool for early diagnosis and epidemiologic surveillance
Authors: Balassiano, Ilana Teruszkin
Vital-Brazil, Juliana Magalhães
Pereira, Martha Maria
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Centro de Referência Nacional para Leptospirose. WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Coleção de Leptospira. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Centro de Referência Nacional para Leptospirose. WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Centro de Referência Nacional para Leptospirose. WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ. Brasil.
Abstract: The aim of this study was to develop an immunocapture polymerase chain reaction (IC-PCR) protocol for leptospirosis. For the standardization of IC-PCR, polyclonal (AS) and monoclonal (MAb) antibodies against different serogroups and serovars of Leptospira were coupled to polystyrene plates. Human sera were artificially contaminated with leptospires and incubated on plates. The bacterial DNA was obtained and used in a multiplex PCR. Sensitivity was tested using sera contaminated with crescent concentrations of leptospires, while specificity was established using sera contaminated with different bacterial genera and sera obtained from patients positive for viral infections. IC-PCR using AS was able to recognize specific serogroups, although some cross-reactions have been observed. No cross-reactions were observed when MAbs were used; however, the sensitivity in this case was lower than that of IC-PCR using AS. IC-PCR proved to be specific to Leptospira and is a promising tool for early diagnosis of leptospirosis, providing additional information about the infecting serovar or serogroup.
Keywords: Leptospirosis
Immunocapture-PCR
Diagnosis
Epidemiology
keywords: Leptospirose
Epidemiologia
Diagnóstico
Reação em Cadeia da Polimerase
Imunocaptura
Issue Date: 2012
Publisher: Elsevier
Citation: BALASSIANO, Ilana Teruszkin; VITAL-BRAZIL, Juliana Magalhães; PEREIRA, Martha Maria. Leptospirosis diagnosis by immunocapture polymerase chain reaction: a new tool for early diagnosis and epidemiologic surveillance. Diagnostic Microbiology and Infectious Disease, v.74, p.11–15, 2012.
DOI: 10.1016/j.diagmicrobio.2012.05.028
ISSN: 0732-8893
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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