| Author | Saldanha, Maíra Garcia | |
| Author | Queiroz, Adriano | |
| Author | Machado, Paulo Roberto Lima | |
| Author | Carvalho, Lucas Pedreira de | |
| Author | Scott, Phillip | |
| Author | Carvalho Filho, Edgar Marcelino | |
| Author | Arruda, Sérgio Marcos | |
| Access date | 2017-05-19T13:35:55Z | |
| Available date | 2017-05-19T13:35:55Z | |
| Document date | 2017 | |
| Citation | SALDANHA, M. G. et al. Characterization of the histopathologic features in patients in the early and late phases of Cutaneous Leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 96, n. 3, p. 645–652, 2017. | pt_BR |
| ISSN | 0002-9637 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18978 | |
| Sponsorship | National Institutes of Health (grant AI088650) and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia—Doenças Tropicais (grant 573839/2008-5). | pt_BR |
| Language | eng | pt_BR |
| Publisher | American Society of Tropical Medicine and Hygiene | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmaniose cutânea | pt_BR |
| Subject in Portuguese | Leishmania (Viannia) braziliensis | pt_BR |
| Subject in Portuguese | Histopatologia | pt_BR |
| Subject in Portuguese | Biopsia | pt_BR |
| Subject in Portuguese | Úlcera cutânea | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Title | Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.4269/ajtmh.16-0539 | |
| Abstract | Cutaneous leishmaniasis (CL), characterized by an ulcerated lesion, is the most common clinical form of human leishmaniasis. Before the ulcer develops, patients infected with Leishmania (Viannia) braziliensis present a small papule at the site of the sandfly bite, referred to as early cutaneous leishmaniasis (E-CL). Two to four weeks later the typical ulcer develops, which is considered here as late CL (L-CL). Although there is a great deal known about T-cell responses in patients with L-CL, there is little information about the in situ inflammatory response in E-CL. Histological sections of skin biopsies from 15 E-CL and 28 L-CL patients were stained by hematoxilin and eosin to measure the area infiltrated by cells, as well as tissue necrosis. Leishmania braziliensis amastigotes, CD4(+), CD8(+), CD20(+), and CD68(+) cells were identified and quantified by immunohistochemistry. The number of amastigotes in E-CL was higher than in L-CL, and the inflammation area was larger in classical ulcers than in E-CL. There was no relationship between the number of parasites and magnitude of the inflammation area, or with the lesion size. However, there was a direct correlation between the number of macrophages and the lesion size in E-CL, and between the number of macrophages and necrotic area throughout the course of the disease. These positive correlations suggest that macrophages are directly involved in the pathology of L. braziliensis-induced lesions. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Subject | Cutaneous Leishmaniasis | pt_BR |
| Subject | Leishmania (Viannia) braziliensis | pt_BR |
| Subject | Histopathology | pt_BR |
| Subject | Biopsies | pt_BR |
| Subject | Cutaneous ulcer | pt_BR |
| Subject | Macrophages | pt_BR |
| DeCS | Leishmaniose cutânea | pt_BR |
| DeCS | Leishmania (Viannia) braziliensis | pt_BR |
| DeCS | Histopatologia | pt_BR |
| DeCS | Biópsia | pt_BR |
| DeCS | Úlcera cutânea | pt_BR |
| DeCS | Macrófagos | pt_BR |
| Embargo date | 2018-01-23 | |
