Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/1899
Title: Activity of physalins purified from Physalis angulata in in vitro and in vivo models of cutaneous leishmaniasis
Authors: Guimarães, Elisalva Teixeira
Lima, Milena da Silva
Santos, Luana A
Ribeiro, Ivone Maria
Tomassini, Therezinha Coelho Barbosa
Santos, Ricardo Ribeiro dos
dosSantos, Washington Luis Conrado
Soares, Milena Botelho Pereira
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: We have previously demonstrated the immunomodulatory effects of physalins, secosteroids purified from Physalis angulata. Here we investigate the antileishmanial activity of physalins in vitro and in vivo in a model of cutaneous leishmaniasis.The antileishmanial activity of physalins B, D and F was tested in Leishmania-infected macrophage cultures. For the in vivo studies, BALB/c mice were infected with Leishmania amazonensis subcutaneously in the ear pinna and treated with physalin F by topical administration.Physalins B and F were able to reduce the percentage of Leishmania-infected macrophages and the intracellular parasite number in vitro at concentrations non-cytotoxic to macrophages. More importantly, topical treatment with physalin F significantly reduced the lesion size, the parasite load and histopathological alterations in BALB/c mice infected with L. amazonensis.Our results demonstrate the potent antileishmanial activity of physalins, especially physalin F, and suggest these molecules as the basis for the development of new therapeutic options for cutaneous leishmaniasis.
Keywords: Infections
Macrophages
Mice
Secosteroids
Therapy
DeCS: Physalis
Secoesteróides
Antiprotozoários
Leishmania mexicana
Leishmaniose Cutânea
Camundongos Endogâmicos BALB C
Issue Date: 2009
Citation: GUIMARÃES, E. T. et al. Activity of physalins purified from Physalis angulata in in vitro and in vivo models of cutaneous leishmaniasis. Journal of Antimicrobial Chemotherapy, v.64, n.1, p.84-87, 2009.
DOI: 10.1093/jac/dkp170
ISSN: 0305-7453
Copyright: open access
Appears in Collections:Farmanguinhos - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Activity of physalins purified from Physalis angulata.pdf262.33 kBAdobe PDFThumbnail
View/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.