Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/1960
Title: An Imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
Authors: Chagas Júnior, Adenizar Delgado das
McBride, Alan John Alexander
Athanazio, Daniel Abensur
Figueira, Cláudio Pereira
Medeiros, Marco Alberto
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
McBride, Flávia Weykamp da Cruz
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, Bahia, Brasil
Abstract: In determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (kappa=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (kappa=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.
DeCS: Leptospira interrogans
Vacinas Bacterianas
Leptospirose
Animais
Sensibilidade e Especificidade
Cricetinae
Issue Date: 2009
Citation: CHAGAS-JUNIOR, A. D. et al. An Imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis. Journal of Medical Microbiology, v. 58, n.12, p.1632–1637, 2009.
DOI: 10.1099/jmm.0.014050-0
ISSN: 0022-2615
Appears in Collections:IGM - Artigos de Periódicos

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