| Author | Ferraz-Carvalho, Rafaela S. | |
| Author | Pereira, Marcela A. | |
| Author | Linhares, Leonardo A. | |
| Author | Lira-Nogueira, Mariane C. | |
| Author | Cavalcanti, Isabella Mf | |
| Author | Santos-Magalhães, Nereide S. | |
| Author | Montenegro, Lílian M. | |
| Access date | 2017-06-28T19:08:16Z | |
| Available date | 2017-06-28T19:08:16Z | |
| Document date | 2016 | |
| Citation | FERRAZ-CARVALHO, R. S. et al. Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 5, p. 330–334, May 2016. | |
| ISSN | 1678-8060 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19653 | |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | MDR-TB | |
| Subject in Portuguese | Atividade antimicobacteriana | |
| Subject in Portuguese | Sinergismo | |
| Subject in Portuguese | Ácido usnic | |
| Subject in Portuguese | Lipossomas | |
| Title | Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates | pt_BR |
| Type | Article | en |
| DOI | 10.1590/0074-02760150454 | pt_BR |
| Abstract | Mycobacterium tuberculosis (Mtb) has acquired resistance and consequently the antibiotic therapeutic options available against this microorganism are limited. In this scenario, the use of usnic acid (UA), a natural compound, encapsulated into liposomes is proposed as a new approach in multidrug-resistant tuberculosis (MDR-TB) therapy. Thus the aim of this study was to evaluate the effect of the encapsulation of UA into liposomes, as well as its combination with antituberculous agents such as rifampicin (RIF) and isoniazid (INH) against MDR-TB clinical isolates. The in vitro antimycobacterial activity of UA-loaded liposomes (UA-Lipo) against MDR-TB was assessed by the microdilution method. The in vitro interaction of UA with antituberculous agents was carried out using checkerboard method. Minimal inhibitory concentration values were 31.25 and 0.98 µg/mL for UA and UA-Lipo, respectively. The results exhibited a synergistic interaction between RIF and UA [fractional inhibitory concentration index (FICI) = 0.31] or UA-Lipo (FICI = 0.28). Regarding INH, the combination of UA or UA-Lipo revealed no marked effect (FICI = 1.30-2.50). The UA-Lipo may be used as a dosage form to improve the antimycobacterial activity of RIF, a first-line drug for the treatment of infections caused by Mtb. | |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil. | |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoepidemiologia. Recife, PE, Brasil. | |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil. | |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro Acadêmico de Vitória. Laboratório de Microbiologia e Imunologia. Vitória de Santo Antão, PE, Brasil. | |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoepidemiologia. Recife, PE, Brasil. | |
| Subject | MDR-TB | |
| Subject | Antimycobacterial activity | |
| Subject | Synergism | |
| Subject | Usnic acid | |
| Subject | Liposomes | |
| DeCS | Mycobacterium tuberculosis / Efeitos de drogas | |
| DeCS | Rifampicina / Farmacologia | |
| DeCS | Antibióticos Antituberculosos / Farmacologia | |
| DeCS | Benzofuranos / Farmacologia | |
| DeCS | Isoniazida / Farmacologia | |
| DeCS | Lipossomas | |
