| Author | Benevenute, Jyan Lucas | |
| Author | Dumler, John Stephen | |
| Author | Ogrzewalska, Maria | |
| Author | Roque, André Luiz Rodrigues | |
| Author | Mello, Victoria Valente Califre | |
| Author | Sousa, Keyla Carstens Marques de | |
| Author | Gonçalves, Luiz Ricardo | |
| Author | D'Andrea, Paulo Sérgio | |
| Author | Lemos, Elba Regina Sampaio de | |
| Author | Machado, Rosangela Zacarias | |
| Author | André, Marcos Rogério | |
| Access date | 2017-06-29T16:28:21Z | |
| Available date | 2017-06-29T16:28:21Z | |
| Document date | 2017 | |
| Citation | BENEVUTE, Jyan Lucas; et al. Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brasil. Ticks and Tick-borne Diseases, v.8, n.4, p.646-656, June 2017. | pt_BR |
| ISSN | 1877-959X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19676 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Roedores sinatrópicos | pt_BR |
| Subject in Portuguese | Ehrlichia | pt_BR |
| Subject in Portuguese | Anaplasma | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Chaperonina 60 | pt_BR |
| Title | Assessment of a quantitative 5' nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ttbdis.2017.04.011 | |
| Abstract | New genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5' nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n=60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas. | pt_BR |
| Affilliation | Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil. | pt_BR |
| Affilliation | Johns Hopkins School of Medicine. Baltimore, Maryland, USA / Uniformed Services University. Bethesda, Maryland, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de e Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de e Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil. | pt_BR |
| Subject | Ehrlichia | pt_BR |
| Subject | Anaplasma | pt_BR |
| Subject | synanthropic rodents | pt_BR |
| Subject | Brazil | pt_BR |
| Subject | genotypes | pt_BR |
| Subject | real-time polymerase chain reaction | pt_BR |
| e-ISSN | 1877-9603 | |
| Embargo date | 2030-01-01 | |
