The present study investigates the effect of multiple immersions on Psorophora albigenu eggs and their development cycle under laboratory conditions. Engorged Ps. albigenu specimens were captured in January 2011 around the Transpantaneira Park Road, Mato Grosso, Brazil, using CO2-baited Centers for Disease Control and Prevention light traps. Psorophora albigenu eggs (n = 1274) were subjected to immersion, with a hatching rate between 49% and 73%. The Williams average showed a peak of 29% hatched eggs in the 3rd immersion; after 4-5 immersions, the effect of additional immersions was generally negligible. The average cycle for both males and females was 5.68 ± 1.00 days. This short development time from the egg stage to adult emergence followed the pattern seen in other species of the genus Psorophora.