| Author | Brodskyn, Claudia Ida | |
| Author | Beverley, Stephen M | |
| Author | Titus, Richard G | |
| Access date | 2017-07-27T18:04:20Z | |
| Available date | 2017-07-27T18:04:20Z | |
| Document date | 2000 | |
| Citation | BRODSKYN, C. I.; BEVERLEY, S. M.; TITUS, R. G. Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro. Clinical Experimenral Immunology, v. 119, n. 2, p. 299-304, 2000. | pt_BR |
| ISSN | 0009-9104 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20433 | |
| Description | Brodskyn, Claudia Ida “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Sponsorship | NIH AI-29955 (R.G.T.), NIH AI 29646 and WHO Special Programme in Tropical Disease Research (S.M.B.). C.B. received a fellowship from CAPES/Fulbright in 1997 and from CAPES-Brasilia | pt_BR |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania major | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Subject in Portuguese | Th1 | pt_BR |
| Subject in Portuguese | Th2 | pt_BR |
| Subject in Portuguese | IL-12 | pt_BR |
| Title | Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro | pt_BR |
| Type | Article | pt_BR |
| Abstract | In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-ts-) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania-infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-ts- did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the first stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia. Departamento de Biointeração. Salvador, BA, Brasil / Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Pathology. Fort Collins, CO | pt_BR |
| Affilliation | Washington University. Department of Molecular Microbiology. St Louis, MO, USA | pt_BR |
| Affilliation | Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Pathology. Fort Collins, CO | pt_BR |
| Subject | Leishmania major | pt_BR |
| Subject | Macrophages | pt_BR |
| Subject | Th1 | pt_BR |
| Subject | Th2 | pt_BR |
| Subject | IL-12 | pt_BR |
