Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20919
Title: Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
Authors: Salomão, Kelly
Souza, Eniuce M. de
Pons, Andrea Henriques
Barbosa, Helene S.
Castro, Solange L. de
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Abstract: The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect on Trypanosoma cruzi trypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets in T. cruzi and its effect on experimental infection of mice. The IC(50)/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL(-1), with no damage to the host cells. In epimastigotes Et-Bra induced alterations in reservosomes, Golgi complex and mitochondrion. These effects were confirmed by flow cytometry analysis. In trypomastigotes, Et-Bra led to the loss of plasma membrane integrity. The in vitro studies indicate that Et-Bra interferes in the functionality of the plasma membrane in trypomastigotes and of reservosomes and mitochondrion in epimastigotes. Acutely infected mice were treated orally with Et-Bra and the parasitemia, mortality and GPT, GOT, CK and urea levels were monitored. The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity. Since Et-Bra was not toxic to the animals, it could be assayed in combination with other drugs. Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation.
Keywords: Trypanosoma cruzi
In vitro
In vivo
Brazilian green propolis
Brazil
keywords: Trypanosoma cruzi
In vitro
In vivo
Própole
Brasil
Issue Date: 2011
Publisher: Hindawi Publishing Corporation
Citation: SALOMÂO, Kelly; et al. Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 185918, 11p, 2011.
DOI: 10.1093/ecam/nep014
ISSN: 1741-427X
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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