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https://www.arca.fiocruz.br/handle/icict/21030
ULTRASTRUCTURAL CHANGES IN CLINICAL AND MICROBIOTA ISOLATES OF KLEBSIELLA PNEUMONIAE CARRIERS OF GENES BLA SHV, BLA TEM, BLA CTX-M, OR BLA KPC WHEN SUBJECT TO β-LACTAM ANTIBIOTICS
Genes, Bacterial
Humans
Klebsiella pneumoniae
drug effects
genetics
isolation & purification
ultrastructure
Microbial Sensitivity Tests
Microbiota
Sequence Analysis, DNA
beta-Lactamases
Antibacterianos
Humanos
Klebsiella pneumoniae
Microbiota
Análise de Sequência de DNA
beta-Lactamases
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil
Abstract
The aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC). Two K. pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that K. pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.
Keywords
Anti-Bacterial AgentsGenes, Bacterial
Humans
Klebsiella pneumoniae
drug effects
genetics
isolation & purification
ultrastructure
Microbial Sensitivity Tests
Microbiota
Sequence Analysis, DNA
beta-Lactamases
DeCS
Genes BacterianosAntibacterianos
Humanos
Klebsiella pneumoniae
Microbiota
Análise de Sequência de DNA
beta-Lactamases
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