Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/22788
Title: Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
Authors: Smircich, Pablo
Eastman, Guillermo
Bispo, Saloe
Duhagon, María Ana
Guerra-Slompo, Eloise P.
Garat, Beatriz
Goldenberg, Samuel
Munroe, David J.
Dallagiovanna, Bruno
Holetz, Fabíola Barbieri
Sotelo-Silveira, Jose
Affilliation: Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay. / Department of Genetics. School of Medicine. Universidad de la República. Montevideo, Uruguay.
Department of Genomics. Instituto de Investigaciones Biológicas Clemente Estable. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay. / Department of Genetics. School of Medicine. Universidad de la República. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Cancer Research Technology Program. Leidos Biomedical Research, Inc. Frederick National Laboratory for Cancer Research. Frederick, USA.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Department of Genomics. Instituto de Investigaciones Biológicas Clemente Estable. Montevideo, Uruguay. / Department of Cell and Molecular Biology. School of Sciences. Universidad de la Republica. Montevideo, Uruguay.
Abstract: Due to the absence of transcription initiation regulation of protein coding genes transcribed by RNA polymerase II, posttranscriptional regulation is responsible for the majority of gene expression changes in trypanosomatids. Therefore, cataloging the abundance of mRNAs (transcriptome) and the level of their translation (translatome) is a key step to understand control of gene expression in these organisms. Here we assess the extent of regulation of the transcriptome and the translatome in the Chagas disease causing agent, Trypanosoma cruzi, in both the non-infective (epimastigote) and infective (metacyclic trypomastigote) insect’s life stages using RNA-seq and ribosome profiling. The observed steady state transcript levels support constitutive transcription and maturation implying the existence of distinctive posttranscriptional regulatory mechanisms controlling gene expression levels at those parasite stages. Meanwhile, the downregulation of a large proportion of the translatome indicates a key role of translation control in differentiation into the infective form. The previously described proteomic data correlate better with the translatomes than with the transcriptomes and translational efficiency analysis shows a wide dynamic range, reinforcing the importance of translatability as a regulatory step. Translation efficiencies for protein families like ribosomal components are diminished while translation of the transialidase virulence factors is upregulated in the quiescent infective metacyclic trypomastigote stage.A large subset of genes is modulated at the translation level in two different stages of Trypanosoma cruzi life cycle. Translation upregulation of virulence factors and downregulation of ribosomal proteins indicates different degrees of control operating to prepare the parasite for an infective life form. Taking together our results show that translational regulation, in addition to regulation of steady state level of mRNA, is a major factor playing a role during the parasite differentiation.
Keywords: Neglected Diseases
Transcriptome
Ribosomes
Gene Expression Regulation
Keywords in spanish: Enfermedades Desatendidas
Ribosomas
Regulación de la Expresión Génica
keywords: Trypanosoma cruzi
DeCS: Doenças Negligenciadas
Transcriptoma
Ribossomos
Regulação da Expressão Gênica
Issue Date: 2015
Publisher: BioMed Central
Citation: SMIRCICH, Pablo et al. Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi. BMC Genomics, v. 16, n. 443, p. 1-14, 2015.
DOI: 10.1186/s12864-015-1563-8
ISSN: 1471-2164
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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