Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23765
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dc.contributor.authorSilva, Everton Fagonde da
dc.contributor.authorMedeiros, Marco Alberto
dc.contributor.authorMcBride, Alan John Alexander
dc.contributor.authorMatsunaga, Jim
dc.contributor.authorEsteves, Gabriela dos Santos
dc.contributor.authorRamos, João Gabriel Rosa
dc.contributor.authorSantos, Cleiton Silva
dc.contributor.authorCroda, Júlio Henrique Rosa
dc.contributor.authorHomma, Akira
dc.contributor.authorDellagostin, Odir Antonio
dc.contributor.authorHaake, David A.
dc.contributor.authorReis, Mitermayer Galvão dos
dc.contributor.authorKo, Albert Icksang
dc.date.accessioned2017-12-22T16:52:56Z
dc.date.available2017-12-22T16:52:56Z
dc.date.issued2007
dc.identifier.citationSILVA, E. F. et al. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, v. 25, p. 6277–6286, 2007.
dc.identifier.issn0264-410X
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/23765
dc.language.isoeng
dc.publisherElsevier
dc.relation.isversionofhttps://www.arca.fiocruz.br/handle/icict/5818
dc.rightsopen access
dc.subject.otherLeptospirose
dc.subject.otherVacina
dc.subject.otherProteína recombinante
dc.subject.otherImunidade
dc.subject.otherAnticorpos
dc.subject.otherHamsters
dc.titleThe terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
dc.typeArticle
dc.identifier.doi10.1016/j.vaccine.2007.05.053
dc.description.abstractenSubunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.
dc.creator.affilliationFundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationVeterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA.
dc.creator.affilliationFundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFederal University of Pelotas. Biotechnology Centre. Pelotas, RGS, Brazil.
dc.creator.affilliationVeterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, United States of America / The David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationWeill Medical College of Cornell University. Division of International Medicine and Infectious Disease. NY, USA.
dc.subject.enLeptospirosis
dc.subject.enSubunit vaccine
dc.subject.enLeptospiral immunoglobulin-like protein
dc.subject.enRecombinant protein
dc.subject.enImmunity
dc.subject.enAntibodies
dc.subject.enHamsters
Appears in Collections:Biomanguinhos - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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