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Title: Methicillin-resistant Staphylococcus aureus in Human Milk
Authors: Novak, Franz Reis
Almeida, João Aprigio Guerra de
Warnken, Márcia. Barbosa.
Ferreira-Carvalho, B. T.
Hagler, A. N.
Affilliation: Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Banco de Leite Humano. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Banco de Leite Humano. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Professor Paulo de Góes.
Abstract: We collected and analyzed 500 samples of human milk, from five Brazilian cities (100 from each) todetect methicillin-resistant strains of Staphylococcus aureus (MRSA) producing enterotoxins. We found57 strains of MRSA, and the mecA gene, responsible for resistance, was detected in all of them using aspecific molecular probe. We examined 40 strains for the presence of four enterotoxins, after selecting asubset that included all strains from each region, except for the largest sample, from which 10 wererandomly selected. Among these two presented enterotoxin B, and growth in human colostrum andtrypicase soy broth. After 5 h of incubation at 37°C, population sizes were already higher than 9.4 x 105UFC/ml and enterotoxin was released into culture medium and colostrum. Our results stress the importanceof hygiene, sanitary measures, and appropriate preservation conditions to avoid the proliferationof S. aureus in human milk.
Keywords: Human Milk
Staphylococcus aureus
DeCS: Resistência a Meticilina
Staphylococcus aureus - efeitos de drogas
Leite Humano - microbiologia
Enterotoxinas - biossíntese
Hibridização Genética
Staphylococcus aureus - genética
Issue Date: 2000
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Citation: NOVAK, F. R. et al. Methicillin-resistant Staphylococcus aureus in Human Milk. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 95, n. 1, p. 29-33, jan./fev. 2000.
DOI: 10.1590/S0074-02762000000100003
ISSN: 0074-0276
Copyright: open access
Appears in Collections:INCQS - Artigos de Periódicos
IFF - Artigos de Periódicos

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