Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24803
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dc.contributor.authorSouza, Hugo Amorim dos Santos de
dc.contributor.authorCorrea, Edmar Henrique Costa
dc.contributor.authorBianco Junior, Cesare
dc.contributor.authorAndrade, Márcia Cristina Ribeiro
dc.contributor.authorLima Junior, Josué da Costa
dc.contributor.authorRiccio, Lilian Rose Pratt
dc.contributor.authorDaniel-Ribeiro, Cláudio Tadeu
dc.contributor.authorTotino, Paulo Renato Rivas
dc.date.accessioned2018-02-11T14:10:00Z
dc.date.available2018-02-11T14:10:00Z
dc.date.issued2017
dc.identifier.citationSOUZA, Hugo Amorim dos Santos de; et al. Detection of signal regulatory protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human monoclonal antibody. Frontiers in Immunology, v.8, Article 1814, 7p. Dec. 2017.
dc.identifier.issn1664-3224
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/24803
dc.language.isoeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subject.otherprimatas não-humanos
dc.subject.otherCitometria de fluxo
dc.subject.otherresposta imune
dc.subject.otherproteína reguladora de sinal α
dc.titleDetection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody
dc.typeArticle
dc.identifier.doi10.3389/fimmu.2017.01814
dc.description.abstractenNon-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as Saimiri sciureus, is still a major factor limiting studies in these models. An alternative strategy to circumvent this obstacle has been the selection of immunological reagents directed to humans, which present cross-reactivity with NHP molecules. In this context and considering the key role of inhibitory immunoreceptors-such as the signal regulatory protein α (SIRPα)-in the regulation of immune responses, in the present study, we attempted to evaluate the ability of anti-human SIRPα monoclonal antibodies to recognize SIRPα in antigen-presenting S. sciureus peripheral blood mononuclear cells (PBMC). As shown by flow cytometry analysis, the profile of anti-SIRPα staining as well as the levels of SIRPα-positive cells in PBMC from S. sciureus were similar to those observed in human PBMC. Furthermore, using anti-SIRPα monoclonal antibody, it was possible to detect a decrease of the SIRPα levels on surface of S. sciureus cells after in vitro stimulation with lipopolysaccharides. Finally, using computed-based analysis, we observed a high degree of conservation of SIRPα across six species of primates and the presence of shared epitopes in the extracellular domain between humans and Saimiri genus that could be targeted by antibodies. In conclusion, we have identified a commercially available anti-human monoclonal antibody that is able to detect SIRPα of S. sciureus monkeys and that, therefore, can facilitate the study of the immunomodulatory role of SIRPα when S. sciureus is used as a model.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Serviço de Primatologia. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
dc.subject.ennon-human primates
dc.subject.enSaimiri sciureus
dc.subject.enimmune response
dc.subject.ensignal regulatory protein α
dc.subject.enflow cytometry
Appears in Collections:ICTB - Artigos de Periódicos
IOC - Artigos de Periódicos

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