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LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR YELLOW FEVER VIRUS OUTBREAK SURVEILLANCE, BRAZIL
Fischer, Carlo et al. | Date Issued: 2017
Author
Fischer, Carlo
Torres, Maria C.
Patel, Pranav
Moreira-Soto, Andres
Gould, Ernest A.
Charrel, Rémi N.
Lamballerie, Xavier de
Nogueira, Rita Maria Ribeiro
Sequeira, Patricia C.
Rodrigues, Cintia D. S.
Kümmerer, Beate M.
Drosten, Christian
Landt, Olfert
Filippis, Ana Maria Bispo de
Drexler, Jan Felix
Affilliation
University of Bonn Medical Centre. Bonn, Germany.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
TIB MOLBIOL. Syntheselabor GmbH. Berlin, Germany.
Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin., Humboldt- Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Germany.
Aix-Marseille University. Marseille, France.
Aix-Marseille University. Marseille, France / Institut Hospitalo Universitaire Méditerranée-Infection. Marseille. France.
Aix-Marseille University. Marseille, France / Institut Hospitalo Universitaire Méditerranée-Infection. Marseille. France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
University of Bonn Medical Centre. Bonn, Germany.
German Centre for Infection Research (DZIF). Germany / Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin., Humboldt- Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Germany.
TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
German Centre for Infection Research (DZIF). Germany / Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin., Humboldt- Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Germany.
Abstract
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.
Keywords in Portuguese
Vírus da Febre Amarela
Surto
Brasil
vacinas
 
Keywords
Yellow Fever Virus
Outbreak
PCR
Brazil
Vaccine
 
Publisher
Centers for Disease Control and Prevention
Citation
FISCHER, Carlo; et al. Lineage-Specific Real-Time Reverse Transcription PCR for Yellow Fever Virus Outbreak Surveillance, Brazil. Emerging Infectious Diseases. v.23, n.11, p.1867-1871, Nov. 2017.
DOI
10.3201/eid2311.171131
ISSN
1080-6040