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dc.contributor.authorNovak, Franz Reis
dc.contributor.authorAlmeida, João Aprigio Guerra de
dc.contributor.authorWarnken, Márcia. Barbosa.
dc.contributor.authorFerreira-Carvalho, B. T.
dc.contributor.authorHagler, A. N.
dc.identifier.citationNOVAK, F. R. et al. Methicillin-resistant Staphylococcus aureus in Human Milk. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 95, n. 1, p. 29-33, jan./fev. 2000.
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dc.rightsopen access
dc.titleMethicillin-resistant Staphylococcus aureus in Human Milk
dc.description.abstractenWe collected and analyzed 500 samples of human milk, from five Brazilian cities (100 from each) todetect methicillin-resistant strains of Staphylococcus aureus (MRSA) producing enterotoxins. We found57 strains of MRSA, and the mecA gene, responsible for resistance, was detected in all of them using aspecific molecular probe. We examined 40 strains for the presence of four enterotoxins, after selecting asubset that included all strains from each region, except for the largest sample, from which 10 wererandomly selected. Among these two presented enterotoxin B, and growth in human colostrum andtrypicase soy broth. After 5 h of incubation at 37°C, population sizes were already higher than 9.4 x 105UFC/ml and enterotoxin was released into culture medium and colostrum. Our results stress the importanceof hygiene, sanitary measures, and appropriate preservation conditions to avoid the proliferationof S. aureus in human milk.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Fernandes Figueira. Banco de Leite Humano. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Fernandes Figueira. Banco de Leite Humano. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Professor Paulo de Góes.
dc.subject.enHuman Milk
dc.subject.enStaphylococcus aureus
dc.subject.decsResistência a Meticilina
dc.subject.decsStaphylococcus aureus - efeitos de drogas
dc.subject.decsLeite Humano - microbiologia
dc.subject.decsEnterotoxinas - biossíntese
dc.subject.decsHibridização Genética
dc.subject.decsStaphylococcus aureus - genética
Appears in Collections:INCQS - Artigos de Periódicos
IFF - Artigos de Periódicos

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