Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/24942
Title: Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
Authors: Marcon, Bruna H.
Holetz, Fabíola B.
Eastman, Guillermo
Origa-Alves, Ana Carolina
Amorós, Mariana Andrea
Aguiar, Alessandra Melo de
Rebelatto, Carmen K.
Brofman, Paulo R. S.
Sotelo-Silveira, Jose
Dallagiovanna, Bruno
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto de Investigaciones Biológicas Clemente Estable, Department of Genomics. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Institute of Biology and Experimental Medicine — National Council of Scientific and Technical Research (IByME-CONICET). Laboratory of Stem Cells. Ciudad Autónoma de Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.
Instituto de Investigaciones Biológicas Clemente Estable, Department of Genomics. Montevideo, Uruguay. / Universidad de la Repúblic. School of Sciences. Department of Cell and Molecular Biology. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Abstract: Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.
Keywords: Adult Stem Cells
Adipogenesis
Ribosomes
Protein Biosynthesis
Mesenchymal Stromal Cells
Keywords in spanish: Células Madre Adultas
Adipogénesis
Ribosomas
Biosíntesis de Proteínas
Células del Estroma Mesenquimatoso
keywords: Células Estaminais Mesenquimais derivadas de Adipose
DeCS: Células-Tronco Adultas
Adipogenia
Ribossomos
Biossíntese de Proteínas
Células Mesenquimais Estromais
Issue Date: 2017
Publisher: Elsevier
Citation: MARCON, Bruna H. et al. Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells. Stem Cell Research, n. 25, p. 191-201, 2017.
ISSN: 1876-7753
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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