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QPCR-HIGH RESOLUTION MELT ANALYSIS FOR DRUG SUSCEPTIBILITY TESTING OF MYCOBACTERIUM LEPRAE DIRECTLY FROM CLINICAL SPECIMENS OF LEPROSY PATIENTS
Araujo, Sergio et al. | Date Issued: 2017
Author
Araujo, Sergio
Goulart, Luiz Ricardo
Truman, Richard W.
Goulart, Isabela Maria B.
Vissa, Varalakshmi
Li, Wei
Matsuoka, Masanori
Suffys, Philip
Fontes, Amanda B.
Rosa, Patricia S.
Scollard, David M.
Williams, Diana L.
Affilliation
Universidade Federal de Uberlândia. Centro Nacional de Referência de Dermatologia Sanitária e Lepra. Uberlândia, MG, Brasil / Universidade Federal de Uberlândia. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Uberlândia, MG, Brasil / U.S. Department of Health and Human Services. Health Resources and Services Administration. Healthcare Systems Bureau. Division National Hansen's Disease Programs. Baton Rouge, Louisiana, USA.
Universidade Federal de Uberlândia. Centro Nacional de Referência de Dermatologia Sanitária e Lepra. Uberlândia, MG, Brasil / Universidade Federal de Uberlândia. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Uberlândia, MG, Brasil / Universidade Federal de Uberlânida. Instituto de Genética e Bioquímica. Uberlândia, MG, Brasil.
U.S. Department of Health and Human Services. Health Resources and Services Administration. Healthcare Systems Bureau. Division National Hansen's Disease Programs. Baton Rouge, Louisiana, USA.
Universidade Federal de Uberlândia. Centro Nacional de Referência de Dermatologia Sanitária e Lepra. Uberlândia, MG, Brasil / Universidade Federal de Uberlândia. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Uberlândia, MG, Brasil.
Good Samaritan Society.Fort Collins, Colorado, USA.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.
National Institute of Infectious Diseases. Leprosy Research Center. Tokyo, Japan.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil / Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil.
Instituto Lauro de Souza Lima, Departmento de Biologia. Bauru, São Paulo, Brasil.
U.S. Department of Health and Human Services. Health Resources and Services Administration. Healthcare Systems Bureau. Division National Hansen's Disease Programs. Baton Rouge, Louisiana, USA.
U.S. Department of Health and Human Services. Health Resources and Services Administration. Healthcare Systems Bureau. Division National Hansen's Disease Programs. Baton Rouge, Louisiana, USA.
Abstract
Real-Time PCR-High Resolution Melting (qPCR-HRM) analysis has been recently described for rapid drug susceptibility testing (DST) of Mycobacterium leprae. The purpose of the current study was to further evaluate the validity, reliability, and accuracy of this assay for M. leprae DST in clinical specimens.
Keywords in Portuguese
Mycobacterium leprae
Hanseníase
Análise em tempo real de PCR
testes rápidos de susceptibilidade a fármacos
 
Keywords
Mycobacterium leprae
Leprosy
Real-Time PCR-High Resolution Melting
drug susceptibility testing
 
Publisher
1935-2735
Citation
ARAUJO, Sergio; et al. qPCR-High resolution melt analysis for drug susceptibility testing of Mycobacterium leprae directly from clinical specimens of leprosy patients. PLoS Negl Trop Dis., v.11, n.6, e0005506, 18p, June 2017.
DOI
10.1371/journal.pntd.0005506
ISSN
1935-2727