Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25198
Full metadata record
DC FieldValue
dc.contributor.authorPereira, Agnes Antônia Sampaio
dc.contributor.authorFerreira, Eduardo de Castro
dc.contributor.authorLima, Ana Cristina Viana Mariano da Rocha
dc.contributor.authorTonelli, Gabriel Barbosa
dc.contributor.authorRêgo, Felipe Dutra
dc.contributor.authorPaglia, Adriano Pereira
dc.contributor.authorAndrade Filho, José Dilermando
dc.contributor.authorPaz, Gustavo Fontes
dc.contributor.authorGontijo, Célia Maria Ferreira
dc.date.accessioned2018-03-08T16:27:31Z
dc.date.available2018-03-08T16:27:31Z
dc.date.issued2017
dc.identifier.citationPEREIRA, Agnes Antônia Sampaio et al. Detection of Leishmania spp in silvatic mammals and isolation of Leishmania (Viannia) braziliensis from Rattus rattus in an endemic area for leishmaniasis in Minas Gerais State, Brazil. PLoS One, v. 12, n. 11, art. e0187704, 2017.
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/25198
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subject.otherLeishmaniose
dc.subject.otherdoenças parasitárias
dc.titleDetection of Leishmania spp in silvatic mammals and isolation of Leishmania (Viannia) braziliensis from Rattus rattus in an endemic area for leishmaniasis in Minas Gerais State, Brazil.
dc.typeArticle
dc.identifier.doi10.1371/journal.pone.0187704
dc.description.abstractenKnowledge of potential reservoirs of Leishmania spp. in an anthropic environment is important so that surveillance and control measures can be implemented. The aim of this study was to investigate the infection by Leishmania in small mammals in an area located in Minas Gerais, Brazil, that undergoes changes in its natural environment and presents autochthonous human cases of cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL). For the capture of the animals, Sherman and Tomahawk traps were used and distributed in the peridomicile of houses with reports of autochthonous cases of CL or VL. Six catches were carried out on two consecutive nights with intervals of two months during one year and samples of spleen, liver, tail skin, ear skin and bone marrow of the animals were obtained. Parasitological and molecular methods were used to detect the infection. Identification of the Leishmania species was performed by PCR RFLPhsp70. Twenty five animals of four species were captured: ten Rattus rattus, nine Didelphis albiventris, five Cerradomys subflavus and one Marmosops incanus. In the PCR-hsp70, five animals were positive (20%). The Leishmania species identified in PCR-RFLPhsp70 were: Leishmania braziliensis in D. albiventris (2), C. subflavus (1) and R. rattus (1) and Leishmania infantum in R. rattus (1). The highest positivity rate for L. braziliensis was obtained in the liver samples. The spleen was the only tissue positive for L. infantum. It was isolated in culture medium L. braziliensis from two samples (liver and spleen) of R. rattus. This is the first record of isolation of L. braziliensis from R. rattus in the southeastern region of Brazil. These results are relevant to the knowledge of the epidemiology of leishmaniasis in the region, mainly in the investigation of the presence of hosts and possible reservoirs of the parasite.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz Mato Grosso do Sul. Campo Grande, MS, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
dc.subject.enLeishmania
dc.subject.enParasitic diseases
dc.subject.enMammals
dc.subject.enLeishmania infantum
dc.subject.enSpleen
dc.subject.enRodents
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Detection of Leishmania spp in silvatic mammals and isolation.pdf1.8 MBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.