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MULTIPLEX PCR-BASED DETECTION OF LEPTOSPIRA IN ENVIRONMENTAL WATER SAMPLES OBTAINED FROM A SLUM SETTLEMENT
Vital-Brazil, Juliana Magalhães et al. | Fecha del documento: 2010
Autor
Vital-Brazil, Juliana Magalhães
Balassiano, Ilana Teruszkin
Oliveira, Fabiano Sutter de
Costa, Alberto Dias de Souza
Hillen, Leandro
Pereira, Martha Maria
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Centro de Referência Nacional para Leptospirose. WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Centro de Referência Nacional para Leptospirose. WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ. Brasil.
Águas do Imperador SA. Petrópolis, RJ, Brasil.
Águas do Imperador SA, Petrópolis, RJ, Brasil
Águas do Imperador SA, Petrópolis, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Centro de Referência Nacional para Leptospirose. WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ. Brasil.
Resumen en ingles
The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
Palabras clave en portugues
Leptospira
Reação em Cadeia da Polimerase Multiplex
amostra de água
 
Palabras clave en ingles
Leptospira spp
multiplex PCR
water sample
 
Editor
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Referencia
VITAL-BRAZIL, Juliana Magalhães; et al. Multiplex PCR-based detection of leptospira in environmental water samples obtained from a slum settlement. Mem Inst Oswaldo Cruz, v.105, n.3, p.353-355, May 2010.
ISSN
0074-0276