Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25294
Title: The effects of the diterpenes isolated from the Brazilian brown algae Dictyota pfaffii and Dictyota menstrualis against the herpes simplex type-1 replicative cycle
Authors: Abrantes, Juliana L.
Barbosa, Jussara
Cavalcanti, Diana
Pereira, Renato C.
Fontes, Carlos L. Frederico
Teixeira, Valeria L.
Souza, Thiago L. Moreno
Paixão, Izabel C. P.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Estrutura e Regulação de Proteínas. Programa de Pós- Graduação em Química Biológica. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Estrutura e Regulação de Proteínas. Programa de Pós- Graduação em Química Biológica. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência para Influenza e Viroses Exantemáticas. Laboratório de Vírus Respiratórios e do Sarampo. Rio de Janeiro, RJ. Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Programa de Pós-Graduação em Neuroimunologia. Niterói, RJ, Brasil.
Abstract: We describe in this paper that the diterpenes 8,10,18-trihydroxy-2,6-dolabelladiene ( 1) and (6 R)-6-hydroxydichotoma-4,14-diene-1,17-dial ( 2), isolated from the marine algae DICTYOTA PFAFFII and D. MENSTRUALIS, respectively, inhibited HSV-1 infection in Vero cells. We initially observed that compounds 1 and 2 inhibited HSV-1 replication in a dose-dependent manner, resulting in EC (50) values of 5.10 and 5.90 microM, respectively, for a multiplicity of infection (MOI) of 5. Moreover, the concentration required to inhibit HSV-1 replication was not cytotoxic, resulting in good selective index (SI) values. Next, we found that compound 1 sustained its anti-herpetic activity even when added to HSV-1-infected cells at 6 h after infection, while compound 2 sustained its activity for up to 3 h after infection, suggesting that these compounds inhibit initial events during HSV-1 replication. We also observed that both compounds were incapable of impairing HSV-1 adsorption and penetration. In addition, the tested molecules could decrease the contents of some HSV-1 early proteins, such as UL-8, RL-1, UL-12, UL-30 and UL-9. Our results suggest that the structures of compounds 1 and 2, Brazilian brown algae diterpenes, might be promising for future antiviral design.
Keywords: Herpes simplex
Diterpenes
Brown algae
Brazil
antiviral
keywords: Diterpenos
Herpes Simples
Feófitas
Brasil
Antivirais
Issue Date: 2010
Publisher: Georg Thieme Verlag
Citation: ABRANTES, Juliana L. et al. The Effects of the Diterpenes Isolated from the Brazilian Brown Algae Dictyota pfaffii and Dictyota menstrualis against the Herpes Simplex Type-1 Replicative Cycle. Planta Med., v.76, p.339–344, 2010.
DOI: 10.1055/s-0029-1186144
ISSN: 0032-0943
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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