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2030-01-01
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- IOC - Artigos de Periódicos [12655]
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EFFECTS OF CARBON AND NITROGEN SOURCES ON THE PROTEOME OF PSEUDOMONAS AERUGINOSA PA1 DURING RHAMNOLIPID PRODUCTION
Proteômica
Fonte de carbono e nitrogênio
Ramnolipídio
Biorremediação
Rhamnolipid
Carbon and nitrogen source
Bioremediation
Proteomics
Autor
Afiliación
Resumen en ingles
The PA1 strain of Pseudomonas aeruginosa isolated from oil waste produces rhamnolipid, a biodegradable
surfactant with applications in several industrial and environmental fields. The metabolic pathway and
genetic regulation of rhamnolipid production in P. aeruginosa are poorly understood. Herein, several proteins
directly or indirectly related to rhamnolipid production and their genetic regulations were identified
by comparative proteomic. We compared the proteome of P. aeruginosa PA1 after fermentation in two
different conditions of carbon and nitrogen sources: condition A allowed rhamnolipid production and
condition B prevented it. Protein extracts from cellular pellets were compared using 2D-PAGE stained
with colloidal Coomassie followed by MALDI-TOF/TOF mass spectrometry. We identified 21 differentially
expressed proteins, including those involved in secretion, quorum sensing, oxidative response and
metabolism.
Palabras clave en portugues
Pseudomonas aeruginosaProteômica
Fonte de carbono e nitrogênio
Ramnolipídio
Biorremediação
Palabras clave en ingles
Pseudomonas aeruginosaRhamnolipid
Carbon and nitrogen source
Bioremediation
Proteomics
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