Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25710
Title: Trypanocidal and cytotoxic activities of essential oils from medicinal plants of Northeast of Brazil
Authors: Borges, Andrezza Raposo
Aires, Juliana Ramos de Albuquerque
Higino, Taciana Mirely Maciel
Medeiros, Maria das Graças Freire de
Citó, Antonia Maria das Graças Lopes
Lopes, José Arimatéia Dantas
Figueiredo, Regina Celia Bressan Queiroz de
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Universidade Federal do Piauí. Campus Universitário Ministro Petrônio Portela-Ininga. Departamento de Química. Teresina, PI, Brazil.
Universidade Federal do Piauí. Campus Universitário Ministro Petrônio Portela-Ininga. Departamento de Química. Teresina, PI, Brazil.
Universidade Federal do Piauí. Campus Universitário Ministro Petrônio Portela-Ininga. Departamento de Química. Teresina, PI, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Abstract: Chagas disease, caused by Trypanosoma cruzi, is an important cause of mortality and morbidity in Latin America. There are no vaccines available, the chemotherapy used to treat this illness has serious side effects and its efficacy on the chronic phase of disease is still a matter of debate. In a search for alternative treatment for Chagas disease, essential oils extracted from traditional medicinal plants Lippia sidoides, Lippia origanoides, Chenopodium ambrosioides, Ocimum gratissimum, Justicia pectorales and Vitex agnus-castus were investigated in vitro for trypanocidal and cytotoxic activities. Essential Oils were extracted by hydrodistillation and submitted to chemical analysis by gas chromatography/mass spectrometry. The concentration of essential oils necessary to inhibit 50% of the epimastigotes or amastigotes growth (IC(50)) and to kill 50% of trypomastigote forms (LC(50)) was estimated. The most prevalent chemical constituents of these essential oils were monoterpenes and sesquiterpenes. All essential oils tested demonstrated an inhibitory effect on the parasite growth and survival. L. sidoides and L. origanoides essential oils were the most effective against trypomastigote and amastigote forms respectively. No significant cytotoxic effects were observed in mouse peritoneal macrophages incubated with essential oils which were more selective against the parasites than mammalian cells. Taken together, our results point towards the use of these essential oils as potential chemotherapeutic agent against T. cruzi.
Keywords: Essential oils
Trypanosoma cruzi
Chagas disease
Chemotherapy
Cytotoxicity
keywords: Óleos essenciais
Trypanosoma cruzi
Doença de Chagas
Quimioterapia
Citotoxicidade
DeCS: Acanthaceae / química
Animais
Brasil
Células Cultivadas
Chenopodium ambrosioides / química
Citotoxinas / efeitos adversos
Citotoxinas / farmacologia
Cromatografia Gasosa-Espectrometria de Massa
Concentração Inibidora 50
Macrófagos Peritoneais / efeitos de drogas
Macrófagos Peritoneais / parasitologia
Ratos
Ratos, endogâmicos BALB C
Ocimum / química
Óleos voláteis / química
Óleos voláteis / isolamento e purificação
Óleos Voláteis / Farmacêuticos
Plantas Medicinais / Química
Tripanocidas Agentes / efeitos adversos
Tripanocidas Agentes / Farmacologia
Trypanosoma cruzi / efeitos de drogas
Verbenaceae / química
Issue Date: 2012
Publisher: Elsevier
Citation: BORGES, A. R. et al. Trypanocidal and cytotoxic activities of essential oils from medicinal plants of Northeast of Brazil. Experimental Parasitology, v. 132, n. 2, p. 123–128, out. 2012.
DOI: 10.1016/j.exppara.2012.06.003
ISSN: 1090-2449
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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