Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26041
Title: Cysteine-stabilised peptide extract of Morinda lucida (Benth) leaf exhibits antimalarial activity and augments antioxidant defense system in P. berghei-infected mice.
Authors: Adebayo, Joseph O.
Adewole, Kayode E.
Krettli, Antoniana Ursine
Affilliation: University of Ilorin. Department of Biochemistry, Faculty of Life Sciences. Ilorin, Nigeria.
University of Ilorin. Department of Biochemistry, Faculty of Life Sciences. Ilorin, Nigeria.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Malaria. Belo Horizonte, MG, Brazil.
Abstract: Ethnopharmacological relevance: Cysteine-stabilised peptides (CSP) are majorly explored for their bioactivities with applications in medicine and agriculture. Morinda lucida leaf is used indigenously for the treatment of malaria; it also contains CSP but the role of CSP in the antimalarial activity of the leaf has not been evaluated. Aim of the study: This study was therefore performed to evaluate the antimalarial activity of partially purified cysteine-stabilised peptide extract (PPCPE) of Morinda lucida leaf and its possible augmentation of the antioxidant systems of liver and erythrocytes in murine malaria. Materials and methods: PPCPE was prepared from Morinda lucida leaf. The activity of PPCPE was evaluated in vitro against Plasmodium falciparum W2 and its cytotoxicity against a BGM kidney cell line. PPCPE was also evaluated for its antimalarial activity and its effects on selected liver and erythrocyte antioxidant parameters in P. berghei NK65-infected mice. Results: PPCPE was not active against P. falciparum W2 (IC50: >50 µg/ml) neither was it cytotoxic (MLD50: >1000 µg/ml). However, PPCPE was active against P. berghei NK65 in vivo, causing 51.52% reduction in parasitaemia at 31.25 mg/Kg body weight on day 4 post-inoculation. PPCPE significantly reduced (P < 0.05) malondialdehyde concentrations in the liver and erythrocyte at higher doses compared to untreated controls. PPCPE increased glutathione concentration and activities of glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase in a dose-dependent manner, which was significant (P < 0.05) at higher doses compared to the untreated controls. Conclusion: The results suggest that PPCPE may require bioactivation in vivo in order to exert its antimalarial effect and that PPCPE may augment the antioxidant defense system to alleviate the reactive oxygen species-mediated complications of malaria.
Keywords: Peptides
Morinda lúcida
Leaf
Antimalarial
Antioxidant
keywords: Peptideos
Morinda lúcida
antimalaricos
Issue Date: 2017
Publisher: Elsevier Sequoia
Citation: ADEBAYO, Joseph Olusegun et al. Cysteine-stabilised peptide extract of Morinda lucida (Benth) leaf exhibits antimalarial activity and augments antioxidant defense system in P. berghei-infected mice. J. Ethnopharmacol., v. 207, p. 118-128, 2017.
DOI: 10.1016/j.jep.2017.06.026
ISSN: 0378-8741
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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