| Author | Lima, Tamara D. da Costa | |
| Author | Moura, Danielle M. N. | |
| Author | Reis, Christian R. S. | |
| Author | Vasconcelos, J. Ronnie C. | |
| Author | Ellis, Louise | |
| Author | Carrington, Mark | |
| Author | Figueiredo, Regina C. B. Q. | |
| Author | Melo Neto, Osvaldo P. de | |
| Access date | 2018-04-27T12:06:31Z | |
| Available date | 2018-04-27T12:06:31Z | |
| Document date | 2010 | |
| Citation | DA COSTA LIMA, T. D. et al. Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners. Eukaryotic Cell, v. 9, n. 10, p. 1484–1494, out. 2010. | pt_BR |
| ISSN | 1535-9786 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26166 | |
| Sponsorship | Este trabalho foi parcialmente financiado por subsídios fornecidos pelas agências de fomento brasileiras FACEPE ( 006/2003-CNPq / PPP ) e CNPq ( 476255 / 2004-0 ). O TDDCL, o DMNM e o CRSR receberam bolsas de estudo da CAPES e FACEPE. O JRCV recebeu uma bolsa PQ (“pesquisador visitante”) da FIOCRUZ. O trabalho no laboratório de M. Carrington é financiado pelo Wellcome Trust, e LE recebeu uma bolsa de estudos financiada pelo BBSRC. | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | RNA | pt_BR |
| Subject in Portuguese | Trypanosoma brucei | pt_BR |
| Subject in Portuguese | eIF4G | pt_BR |
| Title | Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/EC.00148-10 | |
| Abstract | Trypanosomatid protozoans are reliant on posttranscriptional processes to control gene expression. Regulation occurs at the levels of mRNA processing, stability, and translation, events that may require the participation of the poly(A) binding protein (PABP). Here, we have undertaken a functional study of the three distinct Leishmania major PABP (LmPABP) homologues: the previously described LmPABP1; LmPABP2, orthologous to the PABP described from Trypanosoma species; and LmPABP3, unique to Leishmania. Sequence identity between the three PABPs is no greater than 40%. In assays measuring binding to A-rich sequences, LmPABP1 binding was poly(A) sensitive but heparin insensitive; LmPABP2 binding was heparin sensitive and less sensitive to poly(A), compatible with unique substitutions observed in residues implicated in poly(A) binding; and LmPABP3 displayed intermediate properties. All three homologues are simultaneously expressed as abundant cytoplasmic proteins in L. major promastigotes, but only LmPABP1 is present as multiple isoforms. Upon transcription inhibition, LmPABP2 and -3 migrated to the nucleus, while LmPABP1 remained predominantly cytoplasmic. Immunoprecipitation assays showed an association between LmPABP2 and -3. Although the three proteins bound to a Leishmania homologue of the translation initiation factor eukaryotic initiation factor 4G (eIF4G) (LmEIF4G3) in vitro, LmPABP1 was the only one to copurify with native LmEIF4G3 from cytoplasmic extracts. Functionality was tested using RNA interference (RNAi) in Trypanosoma brucei, where both orthologues to LmPABP1 and -2 are required for cellular viability. Our results indicate that these homologues have evolved divergent functions, some of which may be unique to the trypanosomatids, and reinforces a role for LmPABP1 in translation through its interaction with the eIF4G homologue. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | University of Cambridge. Department of Biochemistry. Cambridge, United Kingdom. | pt_BR |
| Affilliation | University of Cambridge. Department of Biochemistry. Cambridge, United Kingdom. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | RNA | pt_BR |
| Subject | Trypanosoma brucei | pt_BR |
| Subject | eIF4G | pt_BR |
| DeCS | Sequência de Aminoácidos | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Encadernação | pt_BR |
| DeCS | Sobrevivência Celular | pt_BR |
| DeCS | Fator de iniciação eucariótica-4G / metabolismo | pt_BR |
| DeCS | Leishmania major / genética | pt_BR |
| DeCS | Leishmania major / crescimento e desenvolvimento | pt_BR |
| DeCS | Leishmania major / metabolismo | pt_BR |
| DeCS | Dados de Sequencia Molecular | pt_BR |
| DeCS | Poly A / metabolismo | pt_BR |
| DeCS | Proteínas de Ligação Poli (A) / química | pt_BR |
| DeCS | Proteínas de Ligação Poli (A) / genética | pt_BR |
| DeCS | Proteínas de Ligação Poli (A) / metabolismo | pt_BR |
| DeCS | Ligação proteica | pt_BR |
| DeCS | Biossíntese de Proteínas | pt_BR |
| DeCS | Interferência de RNA | pt_BR |
| DeCS | RNA mensageiro / metabolismo | pt_BR |
| DeCS | Homologia de Sequência, Aminoácidos | pt_BR |
| DeCS | Trypanosoma brucei brucei / genética | pt_BR |
| DeCS | Trypanosoma brucei brucei / metabolismo | pt_BR |
